Cloned (Comment) | Organism |
---|---|
gene ispH, the IspH domain of the Arabidopsis thaliana enzyme HDR corresponding to the full-length IspH of Escherichia coli fails to complement the Escherichia coli ispH mutant | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Arabidopsis thaliana | |
Iron-sulfur cluster | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Arabidopsis thaliana | - |
isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Arabidopsis thaliana | - |
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q94B35 | gene ispH | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Arabidopsis thaliana | isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Arabidopsis thaliana | dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxy-3-methylbut-2-enyl diphosphate reductase | - |
Arabidopsis thaliana |
HDR | - |
Arabidopsis thaliana |
isoprenoid synthesis H | - |
Arabidopsis thaliana |
ispH | - |
Arabidopsis thaliana |
lysis-tolerant B | - |
Arabidopsis thaliana |
LytB | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | compared with the bacterial IspH, cyanobacterial and plant HDRs all contain an extra N-terminal conserved domain (NCD) that is essential for their function. Tyr72 in the NCD and several plant-specific residues around the central active site are critical for Arabidopsis HDR function | Arabidopsis thaliana |
metabolism | the enzyme catalyzes the last step of the methylerythritol phosphate pathway to synthesize isopentenyl diphosphate and dimethylallyl diphosphate | Arabidopsis thaliana |
additional information | the enzyme contains an extra N-terminal conserved domain (NCD) that is essential for its function. Tyr72 in the NCD and several plant-specific residues around the central active site are critical for Arabidopsis HDR function, homology modeling and substrate docking of the IspH domain of the Arabidopsis enzyme HDR, sequence comparisons, overview | Arabidopsis thaliana |