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Literature summary for 1.17.7.4 extracted from

  • Graewert, T.; Kaiser, J.; Zepeck, F.; Laupitz, R.; Hecht, S.; Amslinger, S.; Schramek, N.; Schleicher, E.; Weber, S.; Haslbeck, M.; Buchner, J.; Rieder, C.; Arigoni, D.; Bacher, A.; Eisenreich, W.; Rohdich, F.
    IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis (2004), J. Am. Chem. Soc., 126, 12847-12855.
    View publication on PubMed
Search for more literature for 1.17.7.4

Application

Application Comment Organism
synthesis recombinant expression of enzyme can be greatly enhanced by coexpression of genes from the isc operon which are required for the assembly of iron-sulfur clusters Escherichia coli

Protein Variants

Protein Variants Comment Organism
C12S decrease in iron content, dramatic decrease in activity Escherichia coli
C197S decrease in iron content, dramatic decrease in activity Escherichia coli
C92S decrease in iron content, dramatic decrease in activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibitory Escherichia coli
Co2+ inhibitory Escherichia coli
Fe2+ inhibitory Escherichia coli
Mg2+ inhibitory Escherichia coli
Mn2+ inhibitory Escherichia coli
Ni2+ inhibitory Escherichia coli
Zn2+ complete inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate pH 8.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Iron (3FE-4S)+ cluster, 2.6 mol of iron per mol of subunit, wild-type, 0.6 mol per mol of subunit, mutant C12S, 0.9 mol per mol of subunit, mutant C92S, 1.4 mol, mutant C197S Escherichia coli
additional information no addition of divalent kations required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+
-
 Escherichia coli dimethylallyl diphosphate + isopentenyl diphosphate + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O ratio of dimethylallyl diphosphate to isopentenyl diphosphate produced is 1 to 6.3 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
ispH
-
 Escherichia coli