Cloned (Comment) | Organism |
---|---|
gene ispH, expression as His-tagged protein in Escherichia coli strain XL1-Blue, construction of a synthetic operon containing ispH, ispG and gepE | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, recombinant enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Escherichia coli | - |
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | Escherichia coli | biosynthesis of terpenes | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | Escherichia coli | investigation of biosynthetic coupling and pattern by expression of involved genes from synthetic operon | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P62623 | gene ispH, formerly lytB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein from Escherichia coli strain XL1-Blue, to homogeneity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Escherichia coli | isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
r | |
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Escherichia coli | dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | biosynthesis of terpenes | Escherichia coli | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r | |
isopentenyl diphosphate + NAD(P)+ + H2O | investigation of biosynthetic coupling and pattern by expression of involved genes from synthetic operon | Escherichia coli | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 36161-36173, recombinant enzyme, DNA sequence determination and electrospray mass spectroscopy | Escherichia coli |
? | x * 37000, recombinant enzyme, SDS-PAGE | Escherichia coli |