Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.5.2 extracted from

  • Yu, C.L.; Kale, Y.; Gopishetty, S.; Louie, T.M.; Subramanian, M.
    A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid (2008), J. Bacteriol., 190, 772-776.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.5.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 Caffeine pH 7.5, 35°C Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE Pseudomonas sp.
40000
-
 1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE Pseudomonas sp.
90000
-
 1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE Pseudomonas sp.
158000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1530
-
 pH 7.5, 35°C Pseudomonas sp.

Storage Stability

Storage Stability Organism
-80°C, 5 mM potassium phosphate buffer pH 7.5, enzyme loses activity after a few days Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caffeine + ubiquinone-0 preferred electron acceptor Pseudomonas sp. trimethyl uric acid + ubiquinol-0 the oxygen atom in trimethyl uric acid is derived exclusively from water ?
additional information enzyme is specific for caffeine and theobromine. No activity with xanthine, 3-methylxanthine, 7-methylxanthine, trimethyl uric acid, and 3,7-dimethyl uric acid Pseudomonas sp. ?
-
 ?
theobromine + ubiquinone-0 46% of the activity with caffeine Pseudomonas sp. ? + ubiquinol-0
-
 ?
theophylline + ubiquinone-0 0.6% of the activity with caffeine Pseudomonas sp. ? + ubiquinol-0
-
 ?

Subunits

Subunits Comment Organism
trimer 1 * 90000 + 1 * 40000 + 1 * 20000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
66
-
 or above, activity increases linearly from 25 to 66°C, which is the highest temperature allowed by the experimental conditions Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 30 min, 77% residual activity Pseudomonas sp.
70
-
 30 min, 11% residual activity Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.167
-
 Caffeine pH 7.5, 35°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 52% of maximum activity Pseudomonas sp.
8
-
 47% of maximum activity Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information enzyme is not NAD(P)+-dependent Pseudomonas sp.
ubiquinone-0 preferred electron acceptor Pseudomonas sp.