Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-[(3-cholamidopropyl)-dimethyl-ammonio]1-propane sulfonate | CHAPS, activation | Bos taurus | |
dithiothreitol | non-physiological cofactor | Bos taurus |
General Stability | Organism |
---|---|
high ionic strength inactivates during purification | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | concentrations up to 30% v/v inhibit enzyme activity up to 88% | Bos taurus | |
additional information | not inhibited by warfarin | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12400 | - |
2 * 12400, SDS-PAGE | Bos taurus |
25000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol | Bos taurus | - |
2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.235 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-70°C, microsomal preparation, stable for months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol | i.e. vitamin K 2,3-epoxide | Bos taurus | 3-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol | i.e. 3-hydroxy-2,3-dihydro-vitamin K | ? | |
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol | - |
Bos taurus | 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 12400, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | 2-mercaptoethanol, reduced GSH, 1,2-ethanediol, or reduced lipoic acid are ineffective as cofactors | Bos taurus |