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Literature summary for 1.17.4.4 extracted from

  • Begent, L.A.; Hill, A.P.; Steventon, G.B.; Hutt, A.J.; Pallister, C.J.; Cowell, D.C.
    Characterization and purification of the vitamin K1 2,3 epoxide reductase system from rat liver (2001), J. Pharm. Pharmacol., 53, 481-486.
    View publication on PubMed
Search for more literature for 1.17.4.4

Inhibitors

Inhibitors Comment Organism Structure
warfarin 0.0017 mM, 50% inhibition; mixed non-competitive inhibition vs. vitamin k 2,3-epoxide, competive inhibition vs. DTT Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00042
-
 dithiothreitol
-
 Rattus norvegicus
0.0013
-
 vitamin K 2,3-epoxide
-
 Rattus norvegicus
0.0016
-
 vitamin K 2,3-epoxide in the presence of 0.0017 mM R,S-warfarin Rattus norvegicus
0.00212
-
 dithiothreitol in the presence of 0.0017 mM R,S-warfarin Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
MonoS, hydroxyapatite, DEAE-Sepharose, Sephacryl S-200, partially purified enzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000007
-
 substrate vitamin K 2,3-epoxide, in the presence of 0.0017 mM R,S-warfarin Rattus norvegicus
0.000017
-
 substrate vitamin K 2,3-epoxide Rattus norvegicus
0.00008
-
 substrate dithiothreitol Rattus norvegicus
0.000137
-
-
 Rattus norvegicus
0.00099
-
 substrate dithiothreitol, in the presence of 0.0017 mM R,S-warfarin Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
vitamin K 2,3-epoxide + dithiothreitol substrate vitamin K 2,3-epoxide, in the presence of 0.0017 mM R,S-warfarin Rattus norvegicus vitamin K + oxidized dithiothreitol
-
 ?

Subunits

Subunits Comment Organism
More complex of at least two components, one possesing catalytic activity for the conversion of vitamin K 2,3-epoxide, second component contains the binding site for the exogenous (DTT) and endogenous disulphide reductant, this thiol-disulfide redox centre is also the site of warfarin binding Rattus norvegicus