Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dATP | stimulation of CTP reduction | Lactobacillus leichmannii | |
dCTP | stimulation of UTP reduction | Lactobacillus leichmannii | |
dGTP | stimulation of ATP reduction | Lactobacillus leichmannii | |
dTTP | stimulation of GTP reduction | Lactobacillus leichmannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69300 | - |
enzyme in 6 M guanidine-HCl, 0.1 M dithiothreitol, equilibrium ultracentrifugation | Lactobacillus leichmannii |
72500 | - |
native enzyme in 0.1 M glycine buffer, pH 9.1, equilibrium ultracentrifugation | Lactobacillus leichmannii |
72600 | - |
maleated enzyme in 0.05 M phosphate buffer, equilibrium ultracentrifugation | Lactobacillus leichmannii |
74500 | - |
enzyme in 6 M guanidine-HCl, 0.1 M dithiothreitol, heated for 20 min at 60°C, equilibrium ultracentrifugation | Lactobacillus leichmannii |
75300 | - |
native enzyme in 0.05 M phosphate buffer, pH 7.0, sedimentation equilibrium method | Lactobacillus leichmannii |
76000 | - |
gel filtration | Lactobacillus leichmannii |
78400 | - |
SDS-PAGE | Lactobacillus leichmannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | Lactobacillus leichmannii | - |
ribonucleoside triphosphate + reduced thioredoxin | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | Lactobacillus leichmannii | - |
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus leichmannii | - |
strain ATCC 7630 | - |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus leichmannii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
- |
Lactobacillus leichmannii |
Storage Stability | Organism |
---|---|
-65°C, no detectable loss of enzyme has been observed during several years of storage of packed bacteria | Lactobacillus leichmannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
Lactobacillus leichmannii | ribonucleoside triphosphate + reduced thioredoxin | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r | |
ribonucleoside triphosphate + reduced thioredoxin + H2O | - |
Lactobacillus leichmannii | 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 78400, SDS-PAGE | Lactobacillus leichmannii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | - |
Lactobacillus leichmannii |