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Literature summary for 1.17.4.1 extracted from

  • Abbouni, B.; Oehlmann, W.; Stolle, P.; Pierik, A.J.; Auling, G.
    Electron paramagnetic resonance (EPR) spectroscopy of the stable-free radical in the native metallo-cofactor of the manganese-ribonucleotide reductase (Mn-RNR) of Corynebacterium glutamicum (2009), Free Radic. Res., 43, 943-950.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dATP positive effector Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
4-methoxyphenol
-
Corynebacterium glutamicum
EDTA
-
Corynebacterium glutamicum
Hydroxyurea
-
Corynebacterium glutamicum
additional information the enzyme is inhibited by radical scavengers Corynebacterium glutamicum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains only 0.06 mol iron per mol of R2F subunit Corynebacterium glutamicum
Mn2+ 0.8 mol manganese per mol of R2F subunit, oragnized in a coupled binuclear centre with paramagnetic ground state or in a weakly coupled binuclear centre with therminally populated paramagnetic excites state which appears as binuclear Mn2+, overview Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, R2F subunit, DSD-PAGE Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
gene nrdF encoding subunit R2F
-
Corynebacterium glutamicum R163
-
gene nrdF encoding subunit R2F
-

Purification (Commentary)

Purification (Comment) Organism
native R2F subunit by 2',5'-ADP affinity and hydrophobic interaction chromatography, followed by two different steps of gel filtration, an further by anion exchange chromnatography for metallo-cofactor analysis Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53
-
subunit R2F Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CDP as substrate Corynebacterium glutamicum ?
-
?
additional information CDP as substrate Corynebacterium glutamicum R163 ?
-
?

Subunits

Subunits Comment Organism
? x * 34000, R2F subunit, DSD-PAGE Corynebacterium glutamicum
More hybrid holoenzyme, consisting of the small manganese-containing R2F subunit and the large catalytic subunit R1E Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
manganese-ribonucleotide reductase
-
Corynebacterium glutamicum
Mn-RNR
-
Corynebacterium glutamicum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.5
-
-
Corynebacterium glutamicum 4-methoxyphenol
1.7
-
-
Corynebacterium glutamicum Hydroxyurea
2.4
-
-
Corynebacterium glutamicum EDTA