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Literature summary for 1.17.4.1 extracted from
- Inhibitory mechanisms of heterocyclic carboxaldehyde thiosemicabazones for two forms of human ribonucleotide reductase (2009), Biochem. Pharmacol., 78, 1178-1185.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged subunits M2, p53R2, and M1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,5-diaminopyridine-2-carboxaldehyde thiosemicarbazone | - |
Homo sapiens | |
| 3-amino-4-methylpyridine-2-carboxaldehyde thiosemicarbazone | - |
Homo sapiens | |
| 3-aminopyridine-2-carboxaldehyde thiosemicarbazone | triapine | Homo sapiens | |
| 3-methyl aminopyridine-2-carboxaldehyde thiosemicarbazone | - |
Homo sapiens | |
| 5-amino-4-morpholinomethylpyridine-2-carboxaldehyde thiosemicarbazone | - |
Homo sapiens | |
| 5-aminopyridine-2-carboxaldehyde thiosemicarbazone | - |
Homo sapiens | |
| 5-hydroxy-4-methyl-1-formylisoquinoline thiosemicarbazone | - |
Homo sapiens | |
| 5-methyl-4-amino-1-formylisoquinoline thiosemicarbazone | - |
Homo sapiens | |
| gemcitabine | - |
Homo sapiens | |
| Hydroxyurea | - |
Homo sapiens | |
| additional information | inhibitory mechanisms of heterocyclic carboxaldehyde thiosemicabazones | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | - |
Homo sapiens | |
| Mg2+ | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged subunits M2, p53R2, and M1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| KB cell | nasopharyngeal carcinoma cells, a gemcitabine-resistant cell line | Homo sapiens | - |
| nasopharyngeal carcinoma cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | dCDP is produced from CDP by the holoenzyme | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | the enzyme consists of M1, M2, and p53R2 subunits in an alphabeta2gamma constellation | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonucleotide reductase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
catalytic activity and ligand binding assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
catalytic activity assay at | Homo sapiens |
| 7.5 | - |
ligand binding assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in DNA replication and damage repair, respectively | Homo sapiens |
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