Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.4.1 extracted from

  • Roshick, C.; Iliffe-Lee, E.R.; McClarty, G.
    Cloning and characterization of ribonucleotide reductase from chlamydia trachomatis (2000), J. Biol. Chem., 275, 38111-38119.
    View publication on PubMed
Search for more literature for 1.17.4.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and amino terminal deleted DELTA1-248 R1 subunit and wild-type, F127Y, Y129F and F127Y/Y129F mutant R2 subunit in Escherichia coli Chlamydia trachomatis

Protein Variants

Protein Variants Comment Organism
F127Y similar CDP reductase activity as wild-type Chlamydia trachomatis
F127Y/Y129F 10-15% of wild-type CDP reductase activity Chlamydia trachomatis
Y129F no CDP reductase activity Chlamydia trachomatis

Inhibitors

Inhibitors Comment Organism Structure
Hydroxyurea
-
 Chlamydia trachomatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Chlamydia trachomatis

Organism

Organism UniProt Comment Textmining
Chlamydia trachomatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type R1 and R2 subunits and F127Y, Y129F and F127Y/Y129F R2 mutant subunits Chlamydia trachomatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.035
-
 recombinant R1 subunit, CDP reduction in the presence of ATP Chlamydia trachomatis
0.048
-
 recombinant DELTA1-248 R1 subunit, CDP reduction in the presence of ATP Chlamydia trachomatis
0.18
-
 recombinant R2 subunit, CDP reduction in the presence of ATP Chlamydia trachomatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonucleoside diphosphate + reduced thioredoxin
-
 Chlamydia trachomatis 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir