Cloned (Comment) | Organism |
---|---|
expression of Y370F and Y370W mutant enzymes in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
Y370F | mutation in R2 subunit, no activity | Mus musculus |
Y370W | mutation in R2 subunit, point mutation does not affect the ability to form a normal diferric iron/tyrosyl radical center, 1.7% of wild-type activity probably due to slow radical transfer | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Y370F and Y370w mutant enzymes | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | - |
Mus musculus | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
More | large subunit R1 contains binding sites for substrates and allosteric effectors, smaller subunit R2 contains non-heme iron and a tyrosyl free-radical | Mus musculus |