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Literature summary for 1.17.4.1 extracted from

  • Rova, U.; Adrait, A.; Potsch, S.; Graslund, A.; Thelander, L.
    Evidence by mutagenesis that Tyr370 of the mouse ribonucleotide reductase R2 protein is the connecting link in the intersubunit radical transfer pathway (1999), J. Biol. Chem., 274, 23746-23751.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of Y370F and Y370W mutant enzymes in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
Y370F mutation in R2 subunit, no activity Mus musculus
Y370W mutation in R2 subunit, point mutation does not affect the ability to form a normal diferric iron/tyrosyl radical center, 1.7% of wild-type activity probably due to slow radical transfer Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Y370F and Y370w mutant enzymes Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonucleoside diphosphate + reduced thioredoxin
-
Mus musculus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
ir

Subunits

Subunits Comment Organism
More large subunit R1 contains binding sites for substrates and allosteric effectors, smaller subunit R2 contains non-heme iron and a tyrosyl free-radical Mus musculus