Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | stimulation of CDP reduction | Corynebacterium ammoniagenes | |
dATP | stimulation of CDP reduction | Corynebacterium ammoniagenes | |
dTTP | stimulation of CDP reduction | Corynebacterium ammoniagenes |
Cloned (Comment) | Organism |
---|---|
expression R1E and R2F subunits in Escherichia coli | Corynebacterium ammoniagenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hydroxyurea | 2 mM, approx. 80% inactivation | Corynebacterium ammoniagenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | EPR-silent Mn bound to the polypeptide chain, approx. 0.5 mol manganese ions/mol of R2F polypeptide | Corynebacterium ammoniagenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37900 | - |
alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence | Corynebacterium ammoniagenes |
81200 | - |
alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence | Corynebacterium ammoniagenes |
160000 | - |
gel filtration | Corynebacterium ammoniagenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium ammoniagenes | O69273 | class Ib reductase | - |
Purification (Comment) | Organism |
---|---|
low-salt precipitation, DEAE-cellulose, Supredex 200, Mono Q | Corynebacterium ammoniagenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.034 | - |
- |
Corynebacterium ammoniagenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | NrdH-redoxin obtained from an overproducing strain, no activity with E. coli thioredoxin | Corynebacterium ammoniagenes | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
trimer | alpha,beta2, 1 * 81200 + 2 * 37900, deduced from nucleotide sequence | Corynebacterium ammoniagenes |