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Literature summary for 1.17.4.1 extracted from

  • Hopper, S.
    Ribonucleotide reductase of rabbit bone marrow (1978), Methods Enzymol., 51, 237-246.
    View publication on PubMed
Search for more literature for 1.17.4.1

General Stability

General Stability Organism
glycerol and ATP required for stabilization Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 5-sulfonate no inhibition in the presence of excess iron Oryctolagus cuniculus
bathophenanthroline sulfonate no effect in the presence of excess iron Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-200, dATP-Sepharose Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00837
-
-
 Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-15°C or -70°C, 10 mM histidine-HCl, pH 7.0, 2 mM dithiotreitol, 40% glycerol, 2 mM ATP, 6 months, no loss of activity Oryctolagus cuniculus
-15°C, 10 mM histidine-HCl, pH 7.0, 2 mM dithiotreitol, 2 mM ATP, 6 months, 80% loss of activity Oryctolagus cuniculus
-70°C, 10 mM histidine-HCl, pH 7.0, 2 mM dithiothreitol, 2 mM ATP, 6 months 60% loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonucleoside diphosphate + reduced thioredoxin
-
 Oryctolagus cuniculus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir