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Literature summary for 1.17.4.1 extracted from
- Ribonucleotide reductase from calf thymus. Purification and properties (1979), Biochemistry, 18, 2941-2948.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-deoxy-2'-azidocytidine diphosphate | thymus enzyme, reversible inhibition | Bos taurus |  |
| 2'-deoxy-2'-azidocytidine diphosphate | inactivation | Escherichia coli | |
| bathophenanthroline sulfonate | 1.5 mM, complete inactivation after 30 min, complete reactivation with FeCl3 | Bos taurus | |
| EDTA | - |
Bos taurus | |
| EDTA | - |
Escherichia coli | |
| Hydroxyurea | 1 mM, approx. 90% inhibition; thymus enzyme, reversible inhibition | Bos taurus | |
| Hydroxyurea | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | - |
Escherichia coli | |
| Iron | nonheme iron is an essential component of the enzyme | Bos taurus | |
| Mg2+ | - |
Escherichia coli | |
| Mg2+ | thymus enzyme: about 50% activity in absence of added Mg2+, optimal Mg2+ concentration varies with concentration of nucleotide effector | Bos taurus | |
| additional information | salt-dependence of calf thymus enzyme: optimal activity in 40 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer, pH 7.6, in the presence of 80-120 mM KCl, precipitation in lower salt concentration, inhibition in higher salt concentration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| streptomycin, ammonium sulfate, DEAE-cellulose, hydroxylapatite, dATP-Sepharose | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
optimal activity in 40 mM 4-(2-hydroxyethyl)-1-piperazineethansulfonic acid, pH 7.6, 80-120 mM KCl | Bos taurus |
| 0.0237 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°, 50 mM Tris-HCl, pH 7.6, 100 mM KCl, several months, no loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ribonucleoside diphosphate + reduced thioredoxin | - |
Escherichia coli | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir | |
| ribonucleoside diphosphate + reduced thioredoxin | - |
Bos taurus | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 37 | reaction rate is about twice as fast at 37°C as the rate at 25°C | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
2'-deoxy-2'-azidocytidine diphosphate | - |
Bos taurus | |
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