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Literature summary for 1.17.4.1 extracted from

  • Hofmann, R.; Feller, W.; Pries, M.; Follmann, H.
    Deoxyribonucleotide biosynthesis in green algae. Purification and characterization of ribonucleotide-diphosphate reductase from Scenedesmus obliquus (1985), Biochim. Biophys. Acta, 832, 98-112.
No PubMed abstract available
Search for more literature for 1.17.4.1

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol high concentrations serve as in vitro hydrogen donor Tetradesmus obliquus
additional information stimulation by effector nucleotides Tetradesmus obliquus
additional information stimulation with various substrates Tetradesmus obliquus
phosphate up to 50 mM, pH 6.7: necessary for activity, decrease of activity at higher values Tetradesmus obliquus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.2 mM, 50% inhibition Tetradesmus obliquus
aurintricarboxylate 0.005 mM, 50% inhibition; oligomeric form Tetradesmus obliquus
dATP
-
 Tetradesmus obliquus
EDTA 10 mM, 50% inhibition Tetradesmus obliquus
Fe2+ 0.2 mM, 50% inhibition Tetradesmus obliquus
Hydroxyurea 1.5 mM, 50% inhibition Tetradesmus obliquus
Mg2+ 2 mM, 50% inhibition Tetradesmus obliquus
Mn2+ 0.2 mM, 50% inhibition Tetradesmus obliquus
N-Hydroxy-alpha-aminoheptanoate 5 mM, 50% inhibition Tetradesmus obliquus
N-Hydroxy-alpha-aminohexanoate 15 mM, 50% inhibition Tetradesmus obliquus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE Tetradesmus obliquus
90000
-
 2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE Tetradesmus obliquus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribonucleoside diphosphate + reduced thioredoxin Tetradesmus obliquus thioredoxin is the physiological reductant 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Tetradesmus obliquus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tetradesmus obliquus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ribonucleoside diphosphate + reduced thioredoxin high concentration of dithiothreitol serves as in vitro hydrogen donor, thioredoxin B of Scenedesmus obliqus and yeast thioredoxin are most effective donors Tetradesmus obliquus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ir
ribonucleoside diphosphate + reduced thioredoxin thioredoxin is the physiological reductant Tetradesmus obliquus 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O
-
 ?

Subunits

Subunits Comment Organism
? 2 * 90000 + x * 75000, most likely 1 75000 Da subunit, SDS-PAGE Tetradesmus obliquus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
 Tetradesmus obliquus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 42 15°C: approx. 27% activity, 42°C: approx. 10% activity Tetradesmus obliquus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Tetradesmus obliquus