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Literature summary for 1.17.4.1 extracted from
- Cloning, overproduction, and purification of the B2 subunit of ribonucleoside-diphosphate reductase (1986), J. Bacteriol., 165, 363-366.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of B2 subunit | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chimeric R2 genes in which C-terminal sequences in Escherichia coli subunit R2 are replaced by C-terminal sequences from the mouse subunit R2 | Escherichia coli |
| recombinant B2 subunit, Sephadex G-25, DEAE Bio gel, Sephadex QAE-50 | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.21 | - |
recombinant subunit B2 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ribonucleoside diphosphate + reduced thioredoxin | - |
Escherichia coli | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |  |
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