Literature summary for 1.17.4.1 extracted from

Berglund, O.
Ribonucleoside diphosphate reductase induced by bacteriophage T4. II. Allosteric regulation of substrate sepecificity and catalytic activity (1972), J. Biol. Chem., 247, 7276-7281.

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Activating Compound

Activating Compound	Comment	Organism	Structure
dATP	stimulation of CDP and UDP reduction	Tequatrovirus T4
dCTP	stimulation of CDP reduction	Tequatrovirus T4
dGTP	stimulation of ADP reduction	Tequatrovirus T4
dTTP	stimulation of GDP reduction	Tequatrovirus T4
E. coli thioredoxin reductase	absolute requirement	Tequatrovirus T4
E. coli thioredoxin reductase	enzyme induced in Escherichia coli by infection with bacteriophage T4	Tequatrovirus T4

Inhibitors

Inhibitors	Comment	Organism	Structure
dATP	inhibition of ADP reduction; inhibition of CDP reduction; inhibition of GDP reduction; inhibition of UDP reduction	Tequatrovirus T4
dGTP	inhibition of ATP- and dATP stimulated CDP reduction	Tequatrovirus T4

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	effect of different nucleoside triphosphates on Km	Tequatrovirus T4
0.04	-	GDP	in the presence of 0.4 mM dTTP as effector	Tequatrovirus T4
0.043	-	CDP	in the presence of 0.002 mM dATP as effector	Tequatrovirus T4
0.048	-	ADP	in the presence of 0.007 mM dGTP as effector	Tequatrovirus T4
0.056	-	CDP	in the presence of 0.5 mM ATP as effector	Tequatrovirus T4
0.057	-	CDP	in the presence of 0.02 mM dTTP as effector	Tequatrovirus T4
0.1	-	UDP	in the presence of 0.3 mM ATP as effector	Tequatrovirus T4
0.3	-	ADP	-	Tequatrovirus T4
0.31	-	CDP	-	Tequatrovirus T4
0.37	-	GDP	-	Tequatrovirus T4
1.2	-	UDP	-	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	-	enzyme induced in Escherichia coli by infection with bacteriophage T4	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + thioredoxin	-	Tequatrovirus T4	2'-dADP + thioredoxin disulfide + H2O	-	r
CDP + thioredoxin	-	Tequatrovirus T4	dCDP + thioredoxin disulfide + H2O	-	r
CDP + thioredoxin	-	Tequatrovirus T4	2'-dCDP + thioredoxin disulfide + H2O	-	r
GDP + thioredoxin	-	Tequatrovirus T4	2'-dGDP + thioredoxin disulfide + H2O	-	r
ribonucleoside diphosphate + reduced thioredoxin	-	Tequatrovirus T4	2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O	-	ir
UDP + reduced thioredoxin	-	Tequatrovirus T4	2'-deoxy-UDP + oxidized thioredoxin + H2O	-	?

Cofactor

Cofactor	Comment	Organism	Structure
T4 thioredoxin	enzyme induced in E. coli by infection with bacteriophage T4	Tequatrovirus T4

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0002	-	dATP	in the presence of 0.15 mM dGTP	Tequatrovirus T4
0.0004	-	dGTP	with 0.001 mM dATP as positive effector	Tequatrovirus T4
0.0004	-	dATP	in the presence of 0.55 mM dTTP	Tequatrovirus T4
0.0005	-	dGTP	with 0.02 mM dATP as positive effector	Tequatrovirus T4
0.0005	-	dATP	in the presence of 0.005 mM dGTP	Tequatrovirus T4
0.0006	-	dATP	in the presence of 0.05 mM dTTP	Tequatrovirus T4
0.0009	-	dGTP	with 0.17 mM ATP as positive effector	Tequatrovirus T4
0.0018	-	dGTP	with 1.7 mM ATP as positive effector	Tequatrovirus T4

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Release 2023.1 (January 2023)