Any feedback?
Literature summary for 1.17.3.2 extracted from
- Purification of xanthine oxidase from bovine milk by affinity chromatography with a novel gel (2015), J. Enzyme Inhib. Med. Chem., 30, 442-447 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-aminobenzamidine | - |
Bos taurus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | maximal activity at ionic strength around 0.4 | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P80457 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 1645fold from milk to homogeneity by ammonium sulfate fracionation and affinity chromatography using a resin with diazotizated p-aminobenzamidine coupled to L-tyrosine, elution by benzamidine | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| milk | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51082.2 | - |
purified native enzyme, pH 7.6, 37°C | Bos taurus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 150000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified native enzyme, 70% activity is retained after 60 min | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
