Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dihydroxyphenyl dodecanoate | sigmoidal inhibition of superoxide anion formation | Bos taurus | |
3,4-dihydroxyphenyl octanoate | - |
Bos taurus | |
butyl caffeate | competitive inhibition of urate and superoxide anion formation | Bos taurus | |
caffeic acid | competitive inhibition of urate and superoxide anion formation | Bos taurus | |
decyl caffeate | competitive inhibition of urate and superoxide anion formation | Bos taurus | |
heptyl caffeate | competitive inhibition of urate and superoxide anion formation | Bos taurus | |
hexyl caffeate | competitive inhibition of urate and superoxide anion formation | Bos taurus | |
methyl caffeate | competitive inhibition of urate and superoxide anion formation | Bos taurus | |
additional information | alkyl caffeates are strong antioxidants and inhibitors of xanthine oxidase. Caffeic acid derivatives equally suppressed O2- generation, and the suppression is stronger than inhibition of xanthine oxidase. Suppression of O2- generation catalyzed by xanthine oxidase with caffeic acid derivatives is not due to enzyme inhibition or O2 - scavenging but due to the reduction of xanthine oxidase molecules. Alkyl caffeates are effective inhibitors of uric acid and O2- catalyzed by xanthine oxidase as well as antioxidants for edible oil | Bos taurus | |
octyl protocatechuate | competitive inhibition of urate and superoxide anion formation | Bos taurus | |
pentyl caffeate | competitive inhibition of urate and superoxide anion formation | Bos taurus | |
propyl caffeate | competitive inhibition of urate and superoxide anion formation | Bos taurus | |
propyl protocatechuate | sigmoidal inhibition of superoxide anion formation | Bos taurus | |
protocatechuic acid | competitive inhibition of urate and superoxide anion formation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P80457 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
milk | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | scavenging activities of 1,1-diphenyl-2-picryhydrazyl (DPPH) radical and O2 generated with phenazine methosulfate (PMS) and NADH,overview | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, superoxid anion generation by xanthine oxidase | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
assay at, superoxide anion generation by xanthine oxidase | Bos taurus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0076 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | heptyl caffeate | |
0.01 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | decyl caffeate | |
0.01 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | pentyl caffeate | |
0.0108 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | hexyl caffeate | |
0.0115 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | caffeic acid | |
0.0115 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | propyl caffeate | |
0.012 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | methyl caffeate | |
0.0125 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | heptyl caffeate | |
0.0164 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | decyl caffeate | |
0.018 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | pentyl caffeate | |
0.019 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | hexyl caffeate | |
0.022 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | protocatechuic acid | |
0.026 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | butyl caffeate | |
0.03 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | propyl caffeate | |
0.0627 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | propyl protocatechuate | |
0.082 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | octyl protocatechuate | |
0.125 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | protocatechuic acid | |
0.125 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | octyl protocatechuate | |
0.125 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | propyl protocatechuate | |
0.143 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | methyl caffeate | |
0.2 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | caffeic acid | |
0.2 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | 3,4-dihydroxyphenyl dodecanoate | |
0.2 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | 3,4-dihydroxyphenyl octanoate | |
92.5 | - |
above, pH 10.0, 25°C, inhibition of urate formation | Bos taurus | 3,4-dihydroxyphenyl dodecanoate | |
110 | - |
pH 10.0, 25°C, inhibition of urate formation | Bos taurus | butyl caffeate |