Inhibitors | Comment | Organism | Structure |
---|---|---|---|
allopurinol | - |
Bos taurus | |
apigenin | binding structure, thermodynamic, and kinetic analysis, computational docking | Bos taurus | |
chrysin | strong reversible competitive inhibition, inhibition mechanism. Chrysin interacts with the amino acid residues Leu648, Phe649, Glu802, Leu873,Ser876, Glu879, Arg880, Phe1009, Thr1010, Val1011 and Phe1013 located within the active cavity of the enzyme, insertion of chrysin into the active site occupying the catalytic center of xanthine oxidase to avoid the entrance of xanthine and causing conformational changes in the enzyme. Binding structure, thermodynamic, and kinetic analysis, computational docking. Molecular modeling of enzyme-drug interaction | Bos taurus | |
additional information | chrysin and its structural analogue apigenin exhibit an additive effect on inhibition of the enzyme, computational docking, overview. Structurally, both chrysin and apigenin possess two-benzene rings, which is very important in the inhibition on the activity of xanthine oxidase, whereas apigenin possesses an extra C-4' hydroxy group which might be the main reason of causing different inhibition compared to chrysin | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P80457 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
milk | - |
Bos taurus | - |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetic analysis | Bos taurus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00126 | - |
pH 7.4, 37°C | Bos taurus | chrysin | |
0.00293 | - |
pH 7.4, 37°C | Bos taurus | allopurinol | |
0.00357 | - |
pH 7.4, 37°C | Bos taurus | apigenin |