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Literature summary for 1.17.3.2 extracted from

  • Lin, S.; Zhang, G.; Liao, Y.; Pan, J.
    Inhibition of chrysin on xanthine oxidase activity and its inhibition mechanism (2015), Int. J. Biol. Macromol., 81, 274-282 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
allopurinol
-
Bos taurus
apigenin binding structure, thermodynamic, and kinetic analysis, computational docking Bos taurus
chrysin strong reversible competitive inhibition, inhibition mechanism. Chrysin interacts with the amino acid residues Leu648, Phe649, Glu802, Leu873,Ser876, Glu879, Arg880, Phe1009, Thr1010, Val1011 and Phe1013 located within the active cavity of the enzyme, insertion of chrysin into the active site occupying the catalytic center of xanthine oxidase to avoid the entrance of xanthine and causing conformational changes in the enzyme. Binding structure, thermodynamic, and kinetic analysis, computational docking. Molecular modeling of enzyme-drug interaction Bos taurus
additional information chrysin and its structural analogue apigenin exhibit an additive effect on inhibition of the enzyme, computational docking, overview. Structurally, both chrysin and apigenin possess two-benzene rings, which is very important in the inhibition on the activity of xanthine oxidase, whereas apigenin possesses an extra C-4' hydroxy group which might be the main reason of causing different inhibition compared to chrysin Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P80457
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Bos taurus
-
milk
-
Bos taurus
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetic analysis Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00126
-
pH 7.4, 37°C Bos taurus chrysin
0.00293
-
pH 7.4, 37°C Bos taurus allopurinol
0.00357
-
pH 7.4, 37°C Bos taurus apigenin