Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.3.2 extracted from

  • Stirpe, F.; Della Corte, E.
    The regulation of rat liver xanthine oxidase: conversion of type D (dehydrogenase) into type O (oxidase) by a thermolabile factor, and reversibility by dithioerythritol (1970), Biochim. Biophys. Acta, 212, 195-197.
    View publication on PubMed

General Stability

General Stability Organism
the enzyme from animal tissues can be interconverted to EC 1.1.1.204, that from liver exists in vivo mainly as the dehydrogenase form, but can be converted into the oxidase form by storage at -20°C, by treatment with proteolytic enzymes or with organic solvents, or by thiol reagents such as Cu2+, N-ethylmaleimide or 4-hydroxymercuribenzoate, the effect of the thiol reagents can be reversed by thiols such as 1,4-dithioerythritol, in other animal tissues the enzyme exists almost entirely as EC 1.1.3.22 but can be converted into the dehydrogenase form by 1,4-dithioerythritol Rattus norvegicus
the enzyme from animal tissues can be interconverted to EC 1.1.1.204, that from liver exists in vivo mainly as the dehydrogenase form, but can be converted into the oxidase form by storage at -20°C, by treatment with proteolytic enzymes or with organic solvents, or by thiol reagents such as Cu2+, N-ethylmaleimide or 4-hydroxymercuribenzoate, the effect of the thiol reagents can be reversed by thiols such as 1,4-dithioerythritol, in other animal tissues the enzyme exists almost entirely as EC 1.1.3.22 but can be converted into the dehydrogenase form by 1,4-dithioerythritol Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-molybdenum protein Rattus norvegicus
Iron iron-molybdenum protein Bos taurus
Molybdenum an iron-molybdenum protein Rattus norvegicus
Molybdenum an iron-molybdenum protein Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme from animal tissues can be interconverted to xanthine dehydrogenase, EC 1.1.1.204, the liver enzyme exists in vivo mainly in its dehydrogenase form, but can be converted into the oxidase form by storage at -20°C, by treatment with proteolytic enzymes or with organic solvents, or by thiol reagents such as Cu2+, N-ethylmaleimide or 4-hydroxymercuribenzoate, the effect of the thiol reagents can be reversed by thiols such as 1,4-dithioerythritol, in other animal tissues the enzyme exists almost entirely as EC 1.1.3.22 but can be converted into the dehydrogenase form by 1,4-dithioerythritol Rattus norvegicus
additional information the enzyme from animal tissues can be interconverted to xanthine dehydrogenase, EC 1.1.1.204, the liver enzyme exists in vivo mainly in its dehydrogenase form, but can be converted into the oxidase form by storage at -20°C, by treatment with proteolytic enzymes or with organic solvents, or by thiol reagents such as Cu2+, N-ethylmaleimide or 4-hydroxymercuribenzoate, the effect of the thiol reagents can be reversed by thiols such as 1,4-dithioerythritol, in other animal tissues the enzyme exists almost entirely as EC 1.1.3.22 but can be converted into the dehydrogenase form by 1,4-dithioerythritol Bos taurus
proteolytic modification
-
Rattus norvegicus
proteolytic modification
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypoxanthine + 2 H2O + 2 O2
-
Rattus norvegicus urate + 2 H2O2
-
?
hypoxanthine + 2 H2O + 2 O2
-
Bos taurus urate + 2 H2O2
-
?
xanthine + H2O + O2 electron acceptor O2 Rattus norvegicus uric acid + H2O2
-
?
xanthine + H2O + O2 electron acceptor O2 Bos taurus uric acid + H2O2
-
?

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Rattus norvegicus
FAD flavoprotein Bos taurus