Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.2.3 extracted from

  • da Silva, S.M.; Pacheco, I.; Pereira, I.A.
    Electron transfer between periplasmic formate dehydrogenase and cytochromes c in Desulfovibrio desulfuricans ATCC 27774 (2012), J. Biol. Inorg. Chem., 17, 831-838.
    View publication on PubMed
Search for more literature for 1.17.2.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
 Ferricytochrome c-553 pH 7.6, 37°C Desulfovibrio desulfuricans
0.0039
-
 ferri-TpIc3 pH 7.6, 37°C Desulfovibrio desulfuricans
0.013
-
 ferri-Ssc pH 7.6, 37°C Desulfovibrio desulfuricans

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Desulfovibrio desulfuricans
-
-

Organism

Organism UniProt Comment Textmining
Desulfovibrio desulfuricans
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.019
-
 FDH specific activity of Desulfovibrio desulfuricans cells grown on 40 mM lactate and 28 mM nitrate as electron acceptors, pH 7.6, 37°C Desulfovibrio desulfuricans
0.034
-
 FDH specific activity of Desulfovibrio desulfuricans cells grown on 40 mM formate and 38 mM sulfate as electron acceptors, pH 7.6, 37°C Desulfovibrio desulfuricans
0.097
-
 FDH specific activity of Desulfovibrio desulfuricans cells grown on 40 mM lactate and 38 mM sulfate as electron acceptors, pH 7.6, 37°C Desulfovibrio desulfuricans
0.14
-
 FDH specific activity of Desulfovibrio desulfuricans cells grown on 40 mM formate and 28 mM nitrate as electron acceptors, pH 7.6, 37°C Desulfovibrio desulfuricans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + ferri-Ssc Ssc i.e. split-Soret cytochrome Desulfovibrio desulfuricans CO2 + ferro-Ssc
-
 ?
formate + ferri-TpIc3 TpIc3 i.e. tetraheme type I cytochrome c3 Desulfovibrio desulfuricans CO2 + ferro-TpIc3
-
 ?
formate + ferricytochrome c-553 highest rate for reduction by FdhABC3, much higher than that observed with other cytochromes i.e. TpIc3, Ssc, NhcA or Dsr Desulfovibrio desulfuricans CO2 + ferrocytochrome c-553
-
 ?
additional information analysis of the Desulfovibrio desulfuricans ATCC 27774 genome shows that no cytochrome c-553 is present in this organism, thus the cytochrome c-553 is not a physiological redox partner of FDH and because cytochrome c-553 has a very high redox potentials in contrast to the low redox potentials of TpIc3 it is more likely that TpIc3 is involved in electron transfer from formate Desulfovibrio desulfuricans ?
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 Desulfovibrio desulfuricans
FdhABC3
-
 Desulfovibrio desulfuricans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.1
-
 ferri-TpIc3 pH 7.6, 37°C Desulfovibrio desulfuricans
27
-
 ferri-Ssc pH 7.6, 37°C Desulfovibrio desulfuricans
239
-
 Ferricytochrome c-553 pH 7.6, 37°C Desulfovibrio desulfuricans

Expression

Organism Comment Expression
Desulfovibrio desulfuricans results indicate that FDH is more highly expressed in formate/nitrate-grown cells and that formate is a preferred electron donor for growth with nitrate, in contrast, the FDH activity of formate/sulfate-grown cells is low up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2100
-
 ferri-Ssc pH 7.6, 37°C Desulfovibrio desulfuricans
4400
-
 ferri-TpIc3 pH 7.6, 37°C Desulfovibrio desulfuricans
88000
-
 Ferricytochrome c-553 pH 7.6, 37°C Desulfovibrio desulfuricans