Protein Variants | Comment | Organism |
---|---|---|
K62E | mutant of cytochrome c553 per site-directed mutagenesis with 3fold increased initial reaction velocity | Desulfovibrio vulgaris |
K62E/K63E | mutant of cytochrome c553 per site-directed mutagenesis with decreased turnover and recognition of the enzyme, but 5fold increased initial reaction velocity | Desulfovibrio vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio vulgaris | - |
structure investigation of wild-type and mutants | - |
Purification (Comment) | Organism |
---|---|
- |
Desulfovibrio vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + ferricytochrome c-553 | - |
Desulfovibrio vulgaris | CO2 + ferrocytochrome c-553 | - |
ir |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.13 | - |
formate | K62E,K63E mutant | Desulfovibrio vulgaris | |
1.85 | - |
formate | - |
Desulfovibrio vulgaris | |
1.92 | - |
formate | K62E mutant | Desulfovibrio vulgaris |