Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.2.2 extracted from

  • Hopper, D.J.; Kaderbhai, M.A.; Marriott, S.A.; Young, M.; Rogozinski, J.
    Cloning, sequencing and heterologous expression of the gene for lupanine hydroxylase, a quinocytochrome c from a Pseudomonas sp. (2002), Biochem. J., 367, 483-489.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Pseudomonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ heme-containing inactive protein in inclusion bodies is renatured and reactivated with pyrroloquinoline quinone and Ca2+ to give active enzyme Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72256
-
1 * 72256, calculated from sequence Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lupanine + pyrroloquinoline quinone + H2O Pseudomonas sp. lupanine is a quinolizidine alkaloid produced by plants of the genus Lupinus. It is metabolized by a Pseudomonas sp. with conversion into 17-hydroxylupanine as the first step. The enzyme involved is induced by growth of the organism on lupanine. cofactors. It catalyses the dehydrogenation of lupanine with the PQQ acting as electron acceptor and the electrons are transferred to the haem before being passed to an external acceptor. This acceptor has not yet been identified but the enzyme rapidly reduces horse heart cytochrome c. Addition of water to the dehydrogenated lupanine gives the hydroxylated product 17-hydroxylupanine + pyrroloquinoline quinol
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q934G0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas sp.

Renatured (Commentary)

Renatured (Comment) Organism
heme-containing inactive protein in inclusion bodies is renatured and reactivated with pyrroloquinoline quinone and Ca2+ to give active enzyme Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:lupanine-grown cell
-
Pseudomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lupanine + pyrroloquinoline quinone + H2O
-
Pseudomonas sp. 17-hydroxylupanine + pyrroloquinoline quinol
-
?
lupanine + pyrroloquinoline quinone + H2O lupanine is a quinolizidine alkaloid produced by plants of the genus Lupinus. It is metabolized by a Pseudomonas sp. with conversion into 17-hydroxylupanine as the first step. The enzyme involved is induced by growth of the organism on lupanine. cofactors. It catalyses the dehydrogenation of lupanine with the PQQ acting as electron acceptor and the electrons are transferred to the haem before being passed to an external acceptor. This acceptor has not yet been identified but the enzyme rapidly reduces horse heart cytochrome c. Addition of water to the dehydrogenated lupanine gives the hydroxylated product Pseudomonas sp. 17-hydroxylupanine + pyrroloquinoline quinol
-
?

Subunits

Subunits Comment Organism
monomer 1 * 72256, calculated from sequence Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
heme
-
Pseudomonas sp.
pyrroloquinoline quinone
-
Pseudomonas sp.