Literature summary for 1.17.2.2 extracted from

  • Stampolidis, P.; Kaderbhai, N.N.; Kaderbhai, M.A.
    Presence and role of a second disulphide bond in recombinant lupanine hydroxylase using site-directed mutagenesis with 143Cys/Ser and 124,143Cys/Ser mutations in Escherichia coli (2012), FEMS Microbiol. Lett., 334, 35-43.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas sp.

Engineering

Protein Variants Comment Organism
C143S mutant displays only 25% activity compared to wild-type Pseudomonas sp.
C124S/C143S mutant displays no activity compared to wild-type Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol dithiothreitol-induced reduction of LH followed by Cd2+ treatment results in significant loss of activity in a dose-dependent manner Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Synonyms

Synonyms Comment Organism
lupanine hydroxylase
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas sp.