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Literature summary for 1.17.1.9 extracted from
- Purification of formaldehyde and formate dehydrogenases from pea seeds by affinity chromatography and S-formylglutathione as the intermediate of formaldehyde metabolism (1979), Arch. Biochem. Biophys., 196, 33-45.
General Stability
| General Stability | Organism |
|---|---|
| rapid freezing in liquid nitrogen gives 75% recovery | Pisum sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
S-formylglutathione | - |
Pisum sativum |  |
| 2.1 | - |
formate | - |
Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42400 | - |
2 * 42400, SDS-PAGE | Pisum sativum |
| 80300 | - |
gel filtration | Pisum sativum |
| 82300 | - |
gel filtration | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.09 | - |
- |
Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, without stabilizer, 85% loss of activity, 7% loss of activity in 33% glycerol solution | Pisum sativum |
| 0°C, 50% loss of activity after 1 month | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl formate + NAD+ | - |
Pisum sativum | ? | - |
? | |
| formate + NAD+ | - |
Pisum sativum | CO2 + NADH + H+ | - |
? | |
| S-formylglutathione + NAD+ | lower KM-value than for formate but maximal activity is only 5.5% of that of formate | Pisum sativum | ? | - |
? | |
| S-formylthioglycolate + NAD+ | - |
Pisum sativum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42400, SDS-PAGE | Pisum sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pisum sativum | |
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Release 2023.1 (January 2023)
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