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Literature summary for 1.17.1.9 extracted from

  • Altas, N.; Aslan, A.; Karatas, E.; Chronopoulou, E.; Labrou, N.; Binay, B.
    Heterologous production of extreme alkaline thermostable NAD+-dependent formate dehydrogenase with wide-range pH activity from Myceliophthora thermophila (2017), Process Biochem., 61, 110-118 .
No PubMed abstract available
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 NADH at pH 6.0 and 35°C Thermothelomyces thermophilus
0.4
-
 CO2 at pH 6.0 and 35°C Thermothelomyces thermophilus
1.2
-
 NAD+ at pH 10.5 and 35°C Thermothelomyces thermophilus
7.2
-
 formate at pH 10.5 and 35°C Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus G2QB71
-
-
Thermothelomyces thermophilus ATCC 42464 G2QB71
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Thermothelomyces thermophilus

Storage Stability

Storage Stability Organism
-20°C, glycine buffer, pH 10.5, 30 days, 40% loss of activity Thermothelomyces thermophilus
-80°C, glycine buffer, pH 10.5, 30 days, 50% loss of activity Thermothelomyces thermophilus
25°C, glycine buffer, pH 10.5, 30 days, complete loss of activity Thermothelomyces thermophilus
4°C, glycine buffer, pH 10.5, 30 days, 40% loss of activity Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + NADH
-
 Thermothelomyces thermophilus formate + NAD+
-
 r
CO2 + NADH
-
 Thermothelomyces thermophilus ATCC 42464 formate + NAD+
-
 r
formate + NAD+
-
 Thermothelomyces thermophilus CO2 + NADH + H+
-
 r
formate + NAD+
-
 Thermothelomyces thermophilus ATCC 42464 CO2 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Thermothelomyces thermophilus

Synonyms

Synonyms Comment Organism
FDH
-
 Thermothelomyces thermophilus
NAD+-dependent formate dehydrogenase
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Thermothelomyces thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 49 about 78% activity at 25°C, 100% activity at 35°C, about 90% activity at 45°C, about 67% activity at 47°C, and about 40% activity at 49°C. No activity at 65°C Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 48 melting temperature is 48°C. The enzyme remains stable for up to 30 min at 25°C Thermothelomyces thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 NADH at pH 6.0 and 35°C Thermothelomyces thermophilus
0.1
-
 CO2 at pH 6.0 and 35°C Thermothelomyces thermophilus
0.3
-
 formate at pH 10.5 and 35°C Thermothelomyces thermophilus
0.76
-
 NAD+ at pH 10.5 and 35°C Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5 the enzyme displays a sharp pH optimum for the reaction with sodium bicarbonate at pH pH 5.5-6.0 Thermothelomyces thermophilus
10.5
-
 the enzyme displays a pH optimum for the conversion of formate anion to carbon dioxide at pH pH 10.5 Thermothelomyces thermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermothelomyces thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Thermothelomyces thermophilus isoelectric focusing
-
 6.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
 formate at pH 10.5 and 35°C Thermothelomyces thermophilus
0.17
-
 NADH at pH 6.0 and 35°C Thermothelomyces thermophilus
0.23
-
 CO2 at pH 6.0 and 35°C Thermothelomyces thermophilus
0.63
-
 NAD+ at pH 10.5 and 35°C Thermothelomyces thermophilus