Literature summary for 1.17.1.9 extracted from

Ranasinghe, C.; Guo, Q.; Sapienza, P.J.; Lee, A.L.; Quinn, D.M.; Cheatum, C.M.; Kohen, A.
Protein mass effects on formate dehydrogenase (2017), J. Am. Chem. Soc., 139, 17405-17413 .

Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	[Candida] boidinii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.021	-	NAD+	at pH 7.5 and 25°C	[Candida] boidinii
2.2	-	formate	at pH 7.5 and 25°C	[Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80620	-	calculated from amino acid sequence	[Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
formate + NAD+	[Candida] boidinii	-	CO2 + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
[Candida] boidinii	A0A0A1EQY0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
formate + NAD+	-	[Candida] boidinii	CO2 + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 40310, electrospray ionization mass spectrometry	[Candida] boidinii

Synonyms

Synonyms	Comment	Organism
FDH	-	[Candida] boidinii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
59.5	-	melting temperature	[Candida] boidinii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.78	-	formate	at pH 7.5 and 25°C	[Candida] boidinii
2.03	-	NAD+	at pH 7.5 and 25°C	[Candida] boidinii

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	[Candida] boidinii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
820	-	formate	at pH 7.5 and 25°C	[Candida] boidinii
97000	-	NAD+	at pH 7.5 and 25°C	[Candida] boidinii

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Release 2023.1 (January 2023)