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Literature summary for 1.17.1.9 extracted from

  • Aslan, A.S.; Valjakka, J.; Ruupunen, J.; Yildirim, D.; Turner, N.J.; Turunen, O.; Binay, B.
    Chaetomium thermophilum formate dehydrogenase has high activity in the reduction of hydrogen carbonate (HCO3-) to formate (2017), Protein Eng. Des. Sel., 30, 47-55.
    View publication on PubMed
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermochaetoides thermophila
expressed in Escherichia coli BL21(DE3) cells [Candida] methylica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 HCO3- at pH 7.0 and 25°C Thermochaetoides thermophila
0.78
-
 HCO3- at pH 7.0 and 25°C [Candida] methylica
3.3
-
 formate at pH 7.0 and 25°C Thermochaetoides thermophila
7.01
-
 formate at pH 7.0 and 25°C [Candida] methylica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ Thermochaetoides thermophila
-
 CO2 + NADH + H+
-
 ?
formate + NAD+ [Candida] methylica
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
-
-
[Candida] methylica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TAGZyme purification system Thermochaetoides thermophila
TAGZyme purification system [Candida] methylica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Thermochaetoides thermophila CO2 + NADH + H+
-
 ?
formate + NAD+
-
 [Candida] methylica CO2 + NADH + H+
-
 ?
formate + NAD+ + OH-
-
 Thermochaetoides thermophila HCO3- + NADH + H+
-
 ?
formate + NAD+ + OH-
-
 [Candida] methylica HCO3- + NADH + H+
-
 ?
HCO3- + NADH
-
 Thermochaetoides thermophila formate + NAD+
-
 ?
HCO3- + NADH
-
 [Candida] methylica formate + NAD+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
 HCO3- at pH 7.0 and 25°C [Candida] methylica
0.023
-
 HCO3- at pH 7.0 and 25°C Thermochaetoides thermophila
1.31
-
 formate at pH 7.0 and 25°C [Candida] methylica
2.039
-
 formate at pH 7.0 and 25°C Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Thermochaetoides thermophila
8
-
-
 [Candida] methylica

pH Range

pH Minimum pH Maximum Comment Organism
5 11 more than 50% activity between pH 5.0 and 11.0 Thermochaetoides thermophila
5.5 9.5 more than 50% activity between pH 5.0 and 11.0 [Candida] methylica

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermochaetoides thermophila
NAD+
-
 [Candida] methylica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
 HCO3- at pH 7.0 and 25°C [Candida] methylica
0.069
-
 HCO3- at pH 7.0 and 25°C Thermochaetoides thermophila
0.187
-
 formate at pH 7.0 and 25°C [Candida] methylica
0.618
-
 formate at pH 7.0 and 25°C Thermochaetoides thermophila