Protein Variants | Comment | Organism |
---|---|---|
F290A | the thermal stability of the mutant increases 1.3fold compared to the wild type enzyme | Glycine max |
F290D | the thermal stability of the mutant increases 61fold compared to the wild type enzyme | Glycine max |
F290E | the thermal stability of the mutant increases 55fold compared to the wild type enzyme | Glycine max |
F290N | the thermal stability of the mutant increases 12.3fold compared to the wild type enzyme | Glycine max |
F290Q | the thermal stability of the mutant increases 5.1fold compared to the wild type enzyme | Glycine max |
F290S | the thermal stability of the mutant increases 4.8fold compared to the wild type enzyme | Glycine max |
F290T | the thermal stability of the mutant increases 5fold compared to the wild type enzyme | Glycine max |
F290Y | the thermal stability of the mutant increases 1.4fold compared to the wild type enzyme | Glycine max |
F311D | the thermal stability of the mutant increases 2.42fold compared to the wild type enzyme | Pseudomonas sp. |
F311N | the thermal stability of the mutant decreases 0.49fold compared to the wild type enzyme | Pseudomonas sp. |
F311S | the thermal stability of the mutant decreases 0.52fold compared to the wild type enzyme | Pseudomonas sp. |
F311Y | the thermal stability of the mutant increases 1.59fold compared to the wild type enzyme | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
NAD+ | mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
0.0091 | - |
NAD+ | mutant enzyme F290S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
0.0109 | - |
NAD+ | mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
0.0117 | - |
NAD+ | mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
0.0128 | - |
NAD+ | mutant enzyme F290D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
0.0133 | - |
NAD+ | wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
0.0137 | - |
NAD+ | mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
0.014 | - |
NAD+ | mutant enzyme F290N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
0.0143 | - |
NAD+ | mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
0.041 | - |
NAD+ | wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
0.055 | - |
NAD+ | mutant enzyme F311Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
0.168 | - |
NAD+ | mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
0.17 | - |
NAD+ | mutant enzyme F311S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
0.23 | - |
NAD+ | mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
0.9 | - |
formate | mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
1.1 | - |
formate | mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
1.2 | - |
formate | mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
1.3 | - |
formate | mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
1.5 | - |
formate | wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
2.9 | - |
formate | mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
3.2 | - |
formate | wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
3.7 | - |
formate | mutant enzyme F311Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
4.1 | - |
formate | mutant enzyme F290S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
4.5 | - |
formate | mutant enzyme F290N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
5 | - |
formate | mutant enzyme F290D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
19.2 | - |
formate | mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
22.7 | - |
formate | mutant enzyme F311S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
29.8 | - |
formate | mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | Glycine max | - |
CO2 + NADH + H+ | - |
? | |
formate + NAD+ | Pseudomonas sp. | - |
CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Glycine max | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | - |
Pseudomonas sp. | CO2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Glycine max |
FDH | - |
Pseudomonas sp. |
NAD+-dependent formate dehydrogenase | - |
Glycine max |
NAD+-dependent formate dehydrogenase | - |
Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
NAD+ | mutant enzyme F290N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
2.9 | - |
NAD+ | wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
3.5 | - |
NAD+ | mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
3.5 | - |
NAD+ | mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
3.8 | - |
NAD+ | mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
4 | - |
NAD+ | mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
4 | - |
NAD+ | mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
4.1 | - |
NAD+ | mutant enzyme F290S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
4.7 | - |
NAD+ | mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
5.1 | - |
NAD+ | mutant enzyme F290D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Glycine max | |
5.4 | - |
NAD+ | mutant enzyme F311S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
5.5 | - |
NAD+ | mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
7.3 | - |
NAD+ | mutant enzyme F311Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. | |
7.3 | - |
NAD+ | wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Glycine max | |
NAD+ | - |
Pseudomonas sp. |