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Literature summary for 1.17.1.9 extracted from
- Characterization of a thermally stable and organic solvent-adaptative NAD+-dependent formate dehydrogenase from Bacillus sp. F1 (2011), J. Appl. Microbiol., 111, 1075-1085.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | the enzyme activity is increased 12% over the control by 1 mM EDTA | Bacillus sp. (in: Bacteria) |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bacillus sp. (in: Bacteria) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | complete inhibition at 1 mM | Bacillus sp. (in: Bacteria) | |
| Cu2+ | 94.2% inhibition at 1 mM | Bacillus sp. (in: Bacteria) | |
| Fe3+ | 83.5% inhibition at 1 mM | Bacillus sp. (in: Bacteria) | |
| additional information | no notable effect on the enzyme activity is observed when adding NH4+ or HPO2- | Bacillus sp. (in: Bacteria) | |
| Ni2+ | 12.4% inhibition at 1 mM | Bacillus sp. (in: Bacteria) | |
| Zn2+ | 55.5% inhibition at 1 mM | Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.091 | - |
NAD+ | in 100 mM Tris-HCl (pH 7.0), at 30°C | Bacillus sp. (in: Bacteria) | |
| 3.5 | - |
NADP+ | in 100 mM Tris-HCl (pH 7.0), at 30°C | Bacillus sp. (in: Bacteria) | |
| 19.6 | - |
formate | with NAD+ as cosubstrate, in 100 mM Tris-HCl (pH 7.0), at 30°C | Bacillus sp. (in: Bacteria) | |
| 39.1 | - |
formate | with NADP+ as cosubstrate, in 100 mM Tris-HCl (pH 7.0), at 30°C | Bacillus sp. (in: Bacteria) | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the enzyme activity is increased 13.8% over the control by 1 mM Ca2+ | Bacillus sp. (in: Bacteria) | |
| Co2+ | the enzyme activity is increased 9.8% over the control by 1 mM Co2+ | Bacillus sp. (in: Bacteria) | |
| Fe2+ | the enzyme activity is increased 49.4% over the control by 1 mM Fe2+ | Bacillus sp. (in: Bacteria) | |
| K+ | the enzyme activity is increased 6.7% over the control by 1 mM K+ | Bacillus sp. (in: Bacteria) | |
| Li+ | the enzyme activity is increased 13.8% over the control by 1 mM Li+ | Bacillus sp. (in: Bacteria) | |
| Mg2+ | the enzyme activity is increased 7.6% over the control by 1 mM Mg2+ | Bacillus sp. (in: Bacteria) | |
| Mn2+ | the enzyme activity is increased 29.8% over the control by 1 mM Mn2+ | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
2 * 44000, calculated from amino acid sequence | Bacillus sp. (in: Bacteria) |
| 45000 | - |
2 * 45000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + NAD+ | Bacillus sp. (in: Bacteria) | the enzyme shows strict substrate specificity for formate | CO2 + NADH + H+ | - |
? | |
| formate + NAD+ | Bacillus sp. (in: Bacteria) F1 | the enzyme shows strict substrate specificity for formate | CO2 + NADH + H+ | - |
? | |
| formate + NADP+ | Bacillus sp. (in: Bacteria) | - |
CO2 + NADPH | - |
? | |
| formate + NADP+ | Bacillus sp. (in: Bacteria) F1 | - |
CO2 + NADPH | - |
? | |
| additional information | Bacillus sp. (in: Bacteria) | the enzyme shows no activity when methanol, ethanol, formaldehyde, sodium acetate, sodium malate, sodium oxalate, sodium lactate, sodium succinate, sodium citrate and sodium nitrate are used as the sole substrate | ? | - |
? | |
| additional information | Bacillus sp. (in: Bacteria) F1 | the enzyme shows no activity when methanol, ethanol, formaldehyde, sodium acetate, sodium malate, sodium oxalate, sodium lactate, sodium succinate, sodium citrate and sodium nitrate are used as the sole substrate | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 3-methyl-1-butanol | the enzyme retains 87% of the initial activity after incubating in 60% (v/v) 3-methyl-1-butanol for 1 h | Bacillus sp. (in: Bacteria) |
| Acetone | the enzyme retains nearly 100% of the initial activity after incubating with up to 60% (v/v) acetone | Bacillus sp. (in: Bacteria) |
| dimethyl sulfoxide | the enzyme retains nearly 100% of the initial activity after incubating with up to 20% (v/v) dimethyl sulfoxide | Bacillus sp. (in: Bacteria) |
| Ethanol | the enzyme retains nearly 100% of the initial activity after incubating with up to 20% (v/v) ethanol | Bacillus sp. (in: Bacteria) |
| isopropanol | the enzyme retains nearly 100% of the initial activity after incubating with up to 60% (v/v) isopropanol | Bacillus sp. (in: Bacteria) |
| Methanol | the enzyme retains nearly 100% of the initial activity after incubating with up to 10% (v/v) methanol | Bacillus sp. (in: Bacteria) |
| n-hexane | the enzyme retains nearly 100% of the initial activity after incubating with up to 20% (v/v) n-hexane | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | D6CJK0 | - |
- |
| Bacillus sp. (in: Bacteria) F1 | D6CJK0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.03 | - |
purified enzyme, in 100 mM Tris-HCl (pH 7.0), at 30°C | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + NAD+ | the enzyme shows strict substrate specificity for formate | Bacillus sp. (in: Bacteria) | CO2 + NADH + H+ | - |
? | |
| formate + NAD+ | the enzyme shows strict substrate specificity for formate | Bacillus sp. (in: Bacteria) F1 | CO2 + NADH + H+ | - |
? | |
| formate + NADP+ | - |
Bacillus sp. (in: Bacteria) | CO2 + NADPH | - |
? | |
| formate + NADP+ | - |
Bacillus sp. (in: Bacteria) F1 | CO2 + NADPH | - |
? | |
| additional information | the enzyme shows no activity when methanol, ethanol, formaldehyde, sodium acetate, sodium malate, sodium oxalate, sodium lactate, sodium succinate, sodium citrate and sodium nitrate are used as the sole substrate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| additional information | the enzyme shows no activity when methanol, ethanol, formaldehyde, sodium acetate, sodium malate, sodium oxalate, sodium lactate, sodium succinate, sodium citrate and sodium nitrate are used as the sole substrate | Bacillus sp. (in: Bacteria) F1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 45000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| homodimer | 2 * 44000, calculated from amino acid sequence | Bacillus sp. (in: Bacteria) |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FDH | - |
Bacillus sp. (in: Bacteria) |
| NAD+-dependent formate dehydrogenase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | enzyme activity increases linearly from 25 to 50°C and reaches the highest at 50°C. The enzyme activity at temperatures ranging from 35 to 60°C is always more than 60% of the maximum activity | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | the enzyme is stable at temperatures below 55°C for 30 min and retains 71% of the initial activity after incubation at 60°C for 30 min. The half-life at 60°C is 52.9 min | Bacillus sp. (in: Bacteria) |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.07 | - |
NADP+ | in 100 mM Tris-HCl (pH 7.0), at 30°C | Bacillus sp. (in: Bacteria) | |
| 6.67 | - |
NAD+ | in 100 mM Tris-HCl (pH 7.0), at 30°C | Bacillus sp. (in: Bacteria) | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | the specific activity at pH ranging from 5.5 to 9.5 is always more than 77% of the maximum activity | Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10.5 | the enzyme retains more than 80% of the activities after incubating in buffers with different pH ranging from 4.5 to 10.5 for 1 h | Bacillus sp. (in: Bacteria) |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | preferred cofactor | Bacillus sp. (in: Bacteria) | |
| NADP+ | - |
Bacillus sp. (in: Bacteria) | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | calculated from amino acid sequence | - |
6.9 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.306 | - |
NADP+ | in 100 mM Tris-HCl (pH 7.0), at 30°C | Bacillus sp. (in: Bacteria) | |
| 73.3 | - |
NAD+ | in 100 mM Tris-HCl (pH 7.0), at 30°C | Bacillus sp. (in: Bacteria) | |
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