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Literature summary for 1.17.1.9 extracted from

  • Bekhouche, M.; Blum, L.; Doumeche, B.
    Ionic liquid-inspired cations covalently bound to formate dehydrogenase improve its stability and activity in ionic liquids (2011), ChemCatChem, 3, 875-882.
No PubMed abstract available
Search for more literature for 1.17.1.9

Inhibitors

Inhibitors Comment Organism Structure
1-methyl-3-methylimidazolium dimethylphosphate adding more than 40% (v/v) of 1-methyl-3-methylimidazolium dimethylphosphate inactivates formate dehydrogenase. In the presence of 30% (v/v) 1-methyl-3-methylimidazolium dimethylphosphate, the catalytic efficiency of the wild type enzyme is reduced by 2.8fold [Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 NAD+ wild type enzyme, in the absence of 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii
0.177
-
 NAD+ wild type enzyme, in the presence of 30% (v/v) 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ [Candida] boidinii
-
 CO2 + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
[Candida] boidinii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
FDH
-
 [Candida] boidinii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
890
-
 NAD+ wild type enzyme, in the absence of 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii
1300
-
 NAD+ wild type enzyme, in the presence of 30% (v/v) 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Candida] boidinii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7333
-
 NAD+ wild type enzyme, in the presence of 30% (v/v) 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii
20600
-
 NAD+ wild type enzyme, in the absence of 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii