Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Clostridium carboxidivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADH | in 0.1 M sodium phosphate (pH 6.8), at 37°C | Clostridium carboxidivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80700 | - |
x * 80700, SDS-PAGE | Clostridium carboxidivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + NADH | Clostridium carboxidivorans | - |
formate + NAD+ | - |
r | |
CO2 + NADH | Clostridium carboxidivorans P7T | - |
formate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium carboxidivorans | E2IQB0 | - |
- |
Clostridium carboxidivorans P7T | E2IQB0 | - |
- |
Purification (Comment) | Organism |
---|---|
His GraviTrap column chromatography | Clostridium carboxidivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + NADH | - |
Clostridium carboxidivorans | formate + NAD+ | - |
r | |
CO2 + NADH | - |
Clostridium carboxidivorans P7T | formate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 80700, SDS-PAGE | Clostridium carboxidivorans |
Synonyms | Comment | Organism |
---|---|---|
FDHH | - |
Clostridium carboxidivorans |
formate dehydrogenase H | - |
Clostridium carboxidivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
NADH | in 0.1 M sodium phosphate (pH 6.8), at 37°C | Clostridium carboxidivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Clostridium carboxidivorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
NADH | in 0.1 M sodium phosphate (pH 6.8), at 37°C | Clostridium carboxidivorans |