Literature summary for 1.17.1.9 extracted from

Alissandratos, A.; Kim, H.K.; Matthews, H.; Hennessy, J.E.; Philbrook, A.; Easton, C.J.
Clostridium carboxidivorans strain P7T recombinant formate dehydrogenase catalyzes reduction of CO2 to formate (2013), Appl. Environ. Microbiol., 79, 741-744.

Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Clostridium carboxidivorans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	NADH	in 0.1 M sodium phosphate (pH 6.8), at 37°C	Clostridium carboxidivorans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80700	-	x * 80700, SDS-PAGE	Clostridium carboxidivorans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CO2 + NADH	Clostridium carboxidivorans	-	formate + NAD+	-	r
CO2 + NADH	Clostridium carboxidivorans P7T	-	formate + NAD+	-	r

Organism

Organism	UniProt	Comment	Textmining
Clostridium carboxidivorans	E2IQB0	-	-
Clostridium carboxidivorans P7T	E2IQB0	-	-

Purification (Commentary)

Purification (Comment)	Organism
His GraviTrap column chromatography	Clostridium carboxidivorans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CO2 + NADH	-	Clostridium carboxidivorans	formate + NAD+	-	r
CO2 + NADH	-	Clostridium carboxidivorans P7T	formate + NAD+	-	r

Subunits

Subunits	Comment	Organism
?	x * 80700, SDS-PAGE	Clostridium carboxidivorans

Synonyms

Synonyms	Comment	Organism
FDHH	-	Clostridium carboxidivorans
formate dehydrogenase H	-	Clostridium carboxidivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.08	-	NADH	in 0.1 M sodium phosphate (pH 6.8), at 37°C	Clostridium carboxidivorans

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Clostridium carboxidivorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.6	-	NADH	in 0.1 M sodium phosphate (pH 6.8), at 37°C	Clostridium carboxidivorans

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Release 2023.1 (January 2023)