Literature summary for 1.17.1.9 extracted from

Shabalin, I.G.; Filippova, E.V.; Polyakov, K.M.; Sadykhov, E.G.; Safonova, T.N.; Tikhonova, T.V.; Tishkov, V.I.; Popov, V.O.
Structures of the apo and holo forms of formate dehydrogenase from the bacterium Moraxella sp. C-1: towards understanding the mechanism of the closure of the interdomain cleft (2009), Acta Crystallogr. Sect. D, 65, 1315-1325.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli TG1 cells	Moraxella sp.

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 2.3 M ammonium sulfate in 0.1 M bis Tris buffer pH 6.5 (for the holoenzyme bound to NAD+ and azide) or 2.2 M ammonium sulfate and 2% (w/v) PEG 400 in 0.1 M HEPES buffer pH 7.5 (for the apoenzyme)	Moraxella sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
azide	-	Moraxella sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08	-	NAD+	in 0.1 M potassium phosphate buffer pH 7.0, at 30°C	Moraxella sp.
7.7	-	formate	in 0.1 M potassium phosphate buffer pH 7.0, at 30°C	Moraxella sp.

Organism

Organism	UniProt	Comment	Textmining
Moraxella sp.	O08375	-	-

Purification (Commentary)

Purification (Comment)	Organism
phenyl Sepharose column chromatography and Sephacryl S-200 gel filtration	Moraxella sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
formate + NAD+	-	Moraxella sp.	CO2 + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
FDH	-	Moraxella sp.
NAD+-dependent formate dehydrogenase	-	Moraxella sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7.3	-	formate	in 0.1 M potassium phosphate buffer pH 7.0, at 30°C	Moraxella sp.

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Moraxella sp.

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Release 2023.1 (January 2023)