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Literature summary for 1.17.1.9 extracted from

  • Watanabe, T.; Fujiwara, T.; Umezawa, T.; Shimada, M.; Hattori, T.
    Cloning of a cDNA encoding a NAD-dependent formate dehydrogenase involved in oxalic acid metabolism from the white-rot fungus Ceriporiopsis subvermispora and its gene expression analysis (2008), FEMS Microbiol. Lett., 279, 64-70.
    View publication on PubMed
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Gelatoporia subvermispora

Inhibitors

Inhibitors Comment Organism Structure
ADP 86% inhibition at 50 mM; 90% inhibition at 50 mM Gelatoporia subvermispora
ATP 45% inhibition at 50 mM; 56% inhibition at 50 mM Gelatoporia subvermispora
Cu2+ 82 inhibition at 1 mM; complete inhibition at 1 mM Gelatoporia subvermispora
Hg2+ 98% inhibition at 1 mM; 98% inhibition at 1 mM Gelatoporia subvermispora
NADH 66% inhibition at 0.12 mM; 73% inhibition at 0.12 mM Gelatoporia subvermispora
NADPH 26% inhibition at 0.24 mM; 58% inhibition at 0.24 mM Gelatoporia subvermispora
Sodium azide 94% inhibition at 1 mM; 99% inhibition at 1 mM Gelatoporia subvermispora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
 formate isozyme FDH2 Gelatoporia subvermispora
4.1
-
 formate isozyme FDH1 Gelatoporia subvermispora
74.6
-
 NAD+ isozyme FDH1 Gelatoporia subvermispora
140
-
 NAD+ isozyme FDH2 Gelatoporia subvermispora

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no divalent metal cation is needed as a cofactor for enzyme activity Gelatoporia subvermispora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39300
-
 predicted from amino acid sequence Gelatoporia subvermispora

Organism

Organism UniProt Comment Textmining
Gelatoporia subvermispora A9ZNT8
-
-
Gelatoporia subvermispora A9ZNT9
-
-
Gelatoporia subvermispora CS105 A9ZNT8
-
-
Gelatoporia subvermispora CS105 A9ZNT9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ column chromatography Gelatoporia subvermispora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+ 100% specificity Gelatoporia subvermispora CO2 + NADH + H+
-
 ?
formate + NAD+ 100% specificity Gelatoporia subvermispora CS105 CO2 + NADH + H+
-
 ?
additional information no activity with malate, lactate, glycolate, pyruvate, and glyoxylate Gelatoporia subvermispora ?
-
 ?
additional information no activity with malate, lactate, glycolate, pyruvate, and glyoxylate Gelatoporia subvermispora CS105 ?
-
 ?

Synonyms

Synonyms Comment Organism
FDB2 isozyme Gelatoporia subvermispora
FDH1 isozyme Gelatoporia subvermispora
NAD-dependent formate dehydrogenase
-
 Gelatoporia subvermispora

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Gelatoporia subvermispora

pI Value

Organism Comment pI Value Maximum pI Value
Gelatoporia subvermispora predicted from amino acid sequence
-
 6.29