Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | adding 5 mM NAD+ in 0.2 M KCl improves the FDH elution and increases the specific FDH activity by 1.38fold as compared to elution with 1 M KCl | [Candida] boidinii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] boidinii | - |
strain ATCC 32195 | - |
Purification (Comment) | Organism |
---|---|
precipitation with polyethyleneimine at 0.04 mg/ml and Sepharose-Procion Blue HERB column chromatography | [Candida] boidinii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
crude extract, at pH 7.4 and 25°C | [Candida] boidinii |
2.63 | - |
after 5.5fold purification, at pH 7.4 and 25°C | [Candida] boidinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
[Candida] boidinii | CO2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
[Candida] boidinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Candida] boidinii |