Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 0.9 mM, 50% inhibition | Methylorubrum extorquens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
NAD+ | pH 7.0, 30°C | Methylorubrum extorquens | |
1.67 | - |
formate | pH 7.0, 30°C | Methylorubrum extorquens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 5 mol nonheme iron per mol of enzyme. The beta-subunit contains a putative binding motif for an iron-sulfur cluster. The alpha-subunit contains at least one iron-sulfur cluster | Methylorubrum extorquens | |
Tungsten | contains 1.8 mol tungsten per mol of enzyme | Methylorubrum extorquens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
alpha1beta1, 1 * 107000 + 1 * 61000, SDS-PAGE, MALDI-TOF analysis | Methylorubrum extorquens |
107000 | - |
alpha1beta1, 1 * 107000 + 1 * 61000, SDS-PAGE, MALDI-TOF analysis | Methylorubrum extorquens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | - |
- |
- |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylorubrum extorquens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
73 | - |
- |
Methylorubrum extorquens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + 2,6-dichlorophenolindophenol | - |
Methylorubrum extorquens | CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
formate + 2,6-dichlorophenolindophenol | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
formate + benzyl viologen | - |
Methylorubrum extorquens | CO2 + reduced benzyl viologen | - |
? | |
formate + benzyl viologen | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | CO2 + reduced benzyl viologen | - |
? | |
formate + NAD+ | - |
Methylorubrum extorquens | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha1beta1, 1 * 107000 + 1 * 61000, SDS-PAGE, MALDI-TOF analysis | Methylorubrum extorquens |
Synonyms | Comment | Organism |
---|---|---|
FDH1 | - |
Methylorubrum extorquens |
tungsten-containing formate dehydrogenase | - |
Methylorubrum extorquens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | in 120 mM potassium phosphate buffer | Methylorubrum extorquens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | contains 0.6 mol of noncovalently bound FMN per mol of enzyme. The beta-subunit contains a putative binding motif for NAD+ | Methylorubrum extorquens | |
molybdopterin | the alpha-subunit contains a putative binding motif for the molybdopterin cofactor | Methylorubrum extorquens | |
NAD+ | the beta-subunit contains a putative binding motif for NAD+. No activity with NADP+ | Methylorubrum extorquens |