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Literature summary for 1.17.1.9 extracted from

  • Laukel, M.; Chistoserdova, L.; Lidstrom, M.E.; Vorholt, J.A.
    The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: purification and properties (2003), Eur. J. Biochem., 270, 325-333.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
azide 0.9 mM, 50% inhibition Methylorubrum extorquens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
NAD+ pH 7.0, 30°C Methylorubrum extorquens
1.67
-
formate pH 7.0, 30°C Methylorubrum extorquens

Metals/Ions

Metals/Ions Comment Organism Structure
Iron contains 5 mol nonheme iron per mol of enzyme. The beta-subunit contains a putative binding motif for an iron-sulfur cluster. The alpha-subunit contains at least one iron-sulfur cluster Methylorubrum extorquens
Tungsten contains 1.8 mol tungsten per mol of enzyme Methylorubrum extorquens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
alpha1beta1, 1 * 107000 + 1 * 61000, SDS-PAGE, MALDI-TOF analysis Methylorubrum extorquens
107000
-
alpha1beta1, 1 * 107000 + 1 * 61000, SDS-PAGE, MALDI-TOF analysis Methylorubrum extorquens

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens
-
-
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methylorubrum extorquens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
73
-
-
Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + 2,6-dichlorophenolindophenol
-
Methylorubrum extorquens CO2 + reduced 2,6-dichlorophenolindophenol
-
?
formate + 2,6-dichlorophenolindophenol
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 CO2 + reduced 2,6-dichlorophenolindophenol
-
?
formate + benzyl viologen
-
Methylorubrum extorquens CO2 + reduced benzyl viologen
-
?
formate + benzyl viologen
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 CO2 + reduced benzyl viologen
-
?
formate + NAD+
-
Methylorubrum extorquens CO2 + NADH + H+
-
?
formate + NAD+
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer alpha1beta1, 1 * 107000 + 1 * 61000, SDS-PAGE, MALDI-TOF analysis Methylorubrum extorquens

Synonyms

Synonyms Comment Organism
FDH1
-
Methylorubrum extorquens
tungsten-containing formate dehydrogenase
-
Methylorubrum extorquens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 in 120 mM potassium phosphate buffer Methylorubrum extorquens

Cofactor

Cofactor Comment Organism Structure
FMN contains 0.6 mol of noncovalently bound FMN per mol of enzyme. The beta-subunit contains a putative binding motif for NAD+ Methylorubrum extorquens
molybdopterin the alpha-subunit contains a putative binding motif for the molybdopterin cofactor Methylorubrum extorquens
NAD+ the beta-subunit contains a putative binding motif for NAD+. No activity with NADP+ Methylorubrum extorquens