Cloned (Comment) | Organism |
---|---|
highly expressed in Escherichia coli cells using an expression plasmid with the pUC ori and tac promoter | Paracoccus sp. 12-A |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
NAD+ | pH 7.0, 30°C, recombinant enzyme | Paracoccus sp. 12-A | |
5.6 | - |
formate | pH 7.0, 30°C, recombinant enzyme | Paracoccus sp. 12-A |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43757 | - |
x * 43757, calculation from nucleotide sequence | Paracoccus sp. 12-A |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus sp. 12-A | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Paracoccus sp. 12-A |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.82 | - |
- |
Paracoccus sp. 12-A |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Paracoccus sp. 12-A | CO2 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43757, calculation from nucleotide sequence | Paracoccus sp. 12-A |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Paracoccus sp. 12-A |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 h, stable | Paracoccus sp. 12-A |
55 | - |
1 h, 75% loss of activity | Paracoccus sp. 12-A |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | - |
Paracoccus sp. 12-A |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Paracoccus sp. 12-A |