Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.1.9 extracted from

  • Shinoda, T.; Satoh, T.; Mineki, S.; Iida, M.; Taguchi, H.
    Cloning, nucleotide sequencing, and expression in Escherichia coli of the gene for formate dehydrogenase of Paracoccus sp. 12-A, a formate-assimilating bacterium (2002), Biosci. Biotechnol. Biochem., 66, 271-276.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
highly expressed in Escherichia coli cells using an expression plasmid with the pUC ori and tac promoter Paracoccus sp. 12-A

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
NAD+ pH 7.0, 30°C, recombinant enzyme Paracoccus sp. 12-A
5.6
-
formate pH 7.0, 30°C, recombinant enzyme Paracoccus sp. 12-A

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43757
-
x * 43757, calculation from nucleotide sequence Paracoccus sp. 12-A

Organism

Organism UniProt Comment Textmining
Paracoccus sp. 12-A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Paracoccus sp. 12-A

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.82
-
-
Paracoccus sp. 12-A

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
Paracoccus sp. 12-A CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 43757, calculation from nucleotide sequence Paracoccus sp. 12-A

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Paracoccus sp. 12-A

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
1 h, stable Paracoccus sp. 12-A
55
-
1 h, 75% loss of activity Paracoccus sp. 12-A

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7
-
Paracoccus sp. 12-A

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Paracoccus sp. 12-A