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Literature summary for 1.17.1.9 extracted from

  • Fedorchuk, V.V.; Galkin, A.G.; Yasny, I.E.; Kulakova, L.B.; Rojkova, A.M.; Filippova, A.A.; Tishkov, V.I.
    Effect of interactions between amino acid residues 43 and 61 on thermal stability of bacterial formate dehydrogenases (2002), Biochemistry, 67, 1145-1151.
    View publication on PubMed
Search for more literature for 1.17.1.9

Protein Variants

Protein Variants Comment Organism
E61K rate constant of thermal inactivation at 54°C, 60°C, 62°C and 65°C is decreased compared to wild-type enzyme Mycolicibacterium vaccae
E61P rate constant of thermal inactivation at 54°C, 60°C, 62°C and 65°C is decreased compared to wild-type enzyme Mycolicibacterium vaccae
E61Q rate constant of thermal inactivation at 54°C, 60°C, 62°C and 65°C is decreased compared to wild-type enzyme Mycolicibacterium vaccae
K61R rate constant of thermal inactivation at 54°C and at 60°C is slightly decreased, at 62°C, and 65°C the constant is increased compared to wild-type enzyme Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Mycolicibacterium vaccae
-
 N10
-
Mycolicibacterium vaccae N10
-
 N10
-
Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Pseudomonas sp. CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Mycolicibacterium vaccae CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Mycolicibacterium vaccae N10 CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
MycFDH
-
 Mycolicibacterium vaccae
PseFDH
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
54
-
 rate constant of thermal inactivation is 0.00000504 per sec for wild-type enzyme and 0.00000463 per sec for mutant enzyme K61R Pseudomonas sp.
54
-
 rate constant of thermal inactivation is 0.0000104 per sec for wild-type enzyme, 0.0000093 per sec for mutant enzyme E61Q, 0.00000754 per sec for mutant enzyme E61P and 0.00000766 per sec for mutant enzyme E61K Mycolicibacterium vaccae
60
-
 rate constant of thermal inactivation is 0.0000755 per sec for wild-type enzyme and 0.000071 per sec for mutant enzyme K61R Pseudomonas sp.
60
-
 rate constant of thermal inactivation is per 0.000336 sec for wild-type enzyme, 0.000174 per sec for mutant enzyme E61Q, 0.000115 per sec for mutant enzyme E61P and 0.000117 per sec for mutant enzyme E61K Mycolicibacterium vaccae
62
-
 rate constant of thermal inactivation is 0.000155 per sec for wild-type enzyme and 0.000212 per sec for mutant enzyme K61R Pseudomonas sp.
62
-
 rate constant of thermal inactivation is per 0.000924 sec for wild-type enzyme, 0.000590 per sec for mutant enzyme E61Q, 0.000335 per sec for mutant enzyme E61P and 0.0004 per sec for mutant enzyme E61K Mycolicibacterium vaccae
63
-
 rate constant of thermal inactivation is 0.000320 per sec for wild-type enzyme and 0.00000463 per sec for mutant enzyme K61R Pseudomonas sp.
65
-
 rate constant of thermal inactivation is 0.000774 per sec for wild-type enzyme and 0.000813 per sec for mutant enzyme K61R Pseudomonas sp.
65
-
 rate constant of thermal inactivation is per 0.002860 sec for wild-type enzyme, 0.00224 per sec for mutant enzyme E61Q, 0.0012 per sec for mutant enzyme E61P and 0.00131 per sec for mutant enzyme E61K Mycolicibacterium vaccae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas sp.
NAD+
-
 Mycolicibacterium vaccae