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Literature summary for 1.17.1.9 extracted from
- Isolation, purification, and partial characterization of formate dehydrogenase from soybean seed (1983), Plant Physiol., 73, 858-859.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0057 | - |
NAD+ | - |
Glycine soja |  |
| 0.6 | - |
formate | - |
Glycine soja | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
2 * 47000, SDS-PAGE | Glycine soja |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine soja | - |
var Beeson | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glycine soja |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| formate + NAD+ = CO2 + NADH + H+ | ordered mechanism | Glycine soja |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.395 | - |
- |
Glycine soja |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM phosphate buffer, pH 7.5, 1 mM EDTA, 2 mM 2-mercaptoethanol, 9 months, enzyme retains 90 to 98% of its activity | Glycine soja |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + NAD+ | - |
Glycine soja | CO2 + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 47000, SDS-PAGE | Glycine soja |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
10 min, stable | Glycine soja |
| 60 | - |
10 min, 88% loss of activity | Glycine soja |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Glycine soja |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | no activity at pH 5.0 or lower, pH 6.0: optimum | Glycine soja |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Glycine soja | |
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