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Literature summary for 1.17.1.9 extracted from

  • Hou, C.T.; Patel, R.N.; Laskin, A.I.; Barnabe, N.
    NAD-linked formate dehydrogenase from methanol-grown Pichia pastoris NRRL-Y-7556 (1982), Arch. Biochem. Biophys., 216, 296-305.
    View publication on PubMed
Search for more literature for 1.17.1.9

General Stability

General Stability Organism
both dithiothreitol, 10 mM, and glycerol, 10%, are required for stability during purification Komagataella pastoris

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Komagataella pastoris
5-Nitro-8-hydroxyquinoline
-
 Komagataella pastoris
CN-
-
 [Candida] boidinii
CN- 1 mM KCN, 50% inhibition Komagataella pastoris
CN-
-
 Pseudomonas sp.
Cu2+
-
 [Candida] boidinii
Cu2+
-
 Komagataella pastoris
Dithionitrobenzoate
-
 Komagataella pastoris
Hg2+
-
 [Candida] boidinii
Hg2+
-
 Komagataella pastoris
iodoacetamide
-
 Komagataella pastoris
N3-
-
 [Candida] boidinii
N3-
-
 Komagataella pastoris
N3-
-
 Pseudomonas sp.
NEM
-
 Komagataella pastoris
p-hydroxymercuribenzoate
-
 Komagataella pastoris
Thiosemicarbazide
-
 Komagataella pastoris
Thiourea
-
 Komagataella pastoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 NAD+
-
 Komagataella pastoris
16
-
 formate
-
 Komagataella pastoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, SDS-PAGE Komagataella pastoris
94000
-
 gel filtration Komagataella pastoris

Organism

Organism UniProt Comment Textmining
Komagataella pastoris
-
 NRRL Y-7556
-
Komagataella pastoris NRRL Y-7556
-
 NRRL Y-7556
-
Methylobacterium organophilum
-
-
-
Methylococcus capsulatus
-
-
-
Methylosinus trichosporium
-
-
-
Ogataea angusta
-
-
-
Pseudomonas sp.
-
-
-
Torulopsis candida
-
 NRRL Y-11419
-
Torulopsis candida NRRL Y-11419
-
 NRRL Y-11419
-
[Candida] boidinii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Komagataella pastoris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.2
-
-
 Komagataella pastoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Pseudomonas sp. CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Komagataella pastoris CO2 + NADH + H+
-
 ?
formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Ogataea angusta CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Torulopsis candida CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Methylococcus capsulatus CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Methylosinus trichosporium CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Methylobacterium organophilum CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Komagataella pastoris NRRL Y-7556 CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Torulopsis candida NRRL Y-11419 CO2 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 [Candida] boidinii
dimer 2 * 47000, SDS-PAGE Komagataella pastoris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 25
-
 Komagataella pastoris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 10 min, stable up to Komagataella pastoris
60
-
 10 min, complete loss of activity Komagataella pastoris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Komagataella pastoris
6.5 7.5
-
 Methylosinus trichosporium

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 pH 5.5: about 25% of maximal activity, pH 10.0: about 40% of maximal activity Komagataella pastoris

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas sp.
NAD+
-
 Komagataella pastoris
NAD+
-
 [Candida] boidinii
NAD+
-
 Ogataea angusta
NAD+
-
 Torulopsis candida
NAD+
-
 Methylococcus capsulatus
NAD+
-
 Methylosinus trichosporium
NAD+
-
 Methylobacterium organophilum