Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.1.9 extracted from

  • Asano, Y.; Sekigawa, T.; Inukai, H.; Nakazawa, A.
    Purification and properties of formate dehydrogenase from Moraxella sp. strain C-1 (1988), J. Bacteriol., 170, 3189-3193.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithio-bis(2-nitrobenzoic acid)
-
 Moraxella sp.
AgNO3 0.01 mM Moraxella sp.
CN-
-
 Moraxella sp.
Hg2+
-
 Moraxella sp.
hydroxylamine
-
 Moraxella sp.
N3-
-
 Moraxella sp.
NEM
-
 Moraxella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 NAD+
-
 Moraxella sp.
0.18
-
 NAD+
-
 Moraxella sp.
10
-
 formate
-
 Moraxella sp.
13
-
 formate
-
 Moraxella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, SDS-PAGE Moraxella sp.
98000
-
 gel filtration Moraxella sp.

Organism

Organism UniProt Comment Textmining
Moraxella sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Moraxella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.97
-
-
 Moraxella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Moraxella sp. CO2 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, SDS-PAGE Moraxella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 5.2-9.0, 1 h, more than 90% of the activity is retained Moraxella sp.
50
-
 pH 9.0, 10 min, about 50% loss of activity Moraxella sp.
55
-
 pH 9.0, 10 min, stable Moraxella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9
-
 Moraxella sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2 9 30°C, 1 h, more than 90% of the activity is retained Moraxella sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Moraxella sp.