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Literature summary for 1.17.1.9 extracted from

  • Izumi, Y.; Kanzaki, H.; Morita, S.; Futazuka, H.; Yamada, H.
    Characterization of crystalline formate dehydrogenase from Candida methanolica (1989), Eur. J. Biochem., 182, 333-341.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
benzaldehyde slight inhibition Candida methanolica
Cd2+ CdCl2 Candida methanolica
CN- NaCN Candida methanolica
Hg2+
-
Candida methanolica
iodoacetamide
-
Candida methanolica
N3-
-
Candida methanolica
Ni2+ NiCl2 Candida methanolica
o-nitrobenzaldehyde slight inhibition Candida methanolica
PCMB
-
Candida methanolica
pyridoxal 5'-phosphate slight inhibition Candida methanolica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
NAD+
-
Candida methanolica
3
-
formate
-
Candida methanolica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, SDS-PAGE Candida methanolica
71000
-
equilibrium sedimentation Candida methanolica
82000
-
velocity sedimentation Candida methanolica
84000
-
gel filtration Candida methanolica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ Candida methanolica one of the key enzymes in the assimilation of C1 compounds, such as methanol CO2 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Candida methanolica
-
-
-

Reaction

Reaction Comment Organism Reaction ID
formate + NAD+ = CO2 + NADH + H+ sequential ordered bi-bi mechanism Candida methanolica

Storage Stability

Storage Stability Organism
-20°C, potassium phosphate buffer, pH 7.0, 45% v/v glycerol, 1.0 mM dithiothreitol, stable for 2 months Candida methanolica
4°C, 0.01 M buffer, 10 days, 50% loss of activity Candida methanolica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
Candida methanolica CO2 + NADH + H+
-
?
formate + NAD+ one of the key enzymes in the assimilation of C1 compounds, such as methanol Candida methanolica CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Candida methanolica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Candida methanolica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
10 min, pH 6.5-9.5, stable up to Candida methanolica
60
-
10 min, 36% loss of activity Candida methanolica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9.5
-
Candida methanolica

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
30°C, 10 min, 61% loss of activity Candida methanolica
6.5 9.5 10 min, 30°C, stable Candida methanolica
10.5
-
30°C, 10 min, 41% loss of activity Candida methanolica

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Candida methanolica