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Literature summary for 1.17.1.9 extracted from

  • Karzanov, V.V.; Bogatsky, Y.A.; Tishkov, V.I.; Egorov, A.M.
    Evidence for the presence of a new NAD+-dependent formate dehydrogenase in Pseudomonas sp. 101 cells grown on a molybdenum-containing medium (1989), FEMS Microbiol. Lett., 60, 197-200.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CN-
-
Pseudomonas sp.
methanol
-
Pseudomonas sp.
N3-
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
NAD+ in presence of formate Pseudomonas sp.
0.62
-
formate in presence of NAD+ Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
280000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
101, strain VKM B1545D
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.43
-
reaction with NAD+ Pseudomonas sp.
4.55
-
reaction with ferricyanide Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, 50% loss of activity ater 1 month Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
Pseudomonas sp. 2 ferrocyanide + NAD+ + H+
-
?
formate + 2,6-dichloroindophenol 33% of the activity with NAD+ Pseudomonas sp. CO2 + reduced 2,6-dichlorophenolindophenol
-
?
formate + ferricyanide
-
Pseudomonas sp. CO2 + ferrocyanide
-
?
formate + NAD+
-
Pseudomonas sp. CO2 + NADH + H+
-
?
NADH + 2,6-dichloroindophenol
-
Pseudomonas sp. NAD+ + reduced 2,6-dichlorophenolindophenol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
1 min, 50% loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas sp.