Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | - |
Pseudomonas sp. | |
methanol | - |
Pseudomonas sp. | |
N3- | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
NAD+ | in presence of formate | Pseudomonas sp. | |
0.62 | - |
formate | in presence of NAD+ | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
280000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
101, strain VKM B1545D | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.43 | - |
reaction with NAD+ | Pseudomonas sp. |
4.55 | - |
reaction with ferricyanide | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 50% loss of activity ater 1 month | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Pseudomonas sp. | 2 ferrocyanide + NAD+ + H+ | - |
? | |
formate + 2,6-dichloroindophenol | 33% of the activity with NAD+ | Pseudomonas sp. | CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
formate + ferricyanide | - |
Pseudomonas sp. | CO2 + ferrocyanide | - |
? | |
formate + NAD+ | - |
Pseudomonas sp. | CO2 + NADH + H+ | - |
? | |
NADH + 2,6-dichloroindophenol | - |
Pseudomonas sp. | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 min, 50% loss of activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. |