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Literature summary for 1.17.1.9 extracted from

  • Mueller, U.; Willnow, P.; Ruschig, U.; Hoepner, T.
    Formate dehydrogenase from Pseudomonas oxalaticus (1978), Eur. J. Biochem., 83, 485-498.
    View publication on PubMed
Search for more literature for 1.17.1.9

Inhibitors

Inhibitors Comment Organism Structure
CN- 0.25 mM Cupriavidus oxalaticus
N3- 0.25 mM. Inhibition is partly overcome by addition of formate or NAD+ Cupriavidus oxalaticus
NADH inhibition is partly overcome by addition of formate or NAD+ Cupriavidus oxalaticus
NO3- 0.25 mM. Inhibition is partly overcome by addition of formate or NAD+ Cupriavidus oxalaticus
p-hydroxymercuribenzoate 0.25 mM Cupriavidus oxalaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 2,6-dichlorophenolindophenol formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
0.042
-
 2,6-dichlorophenolindophenol formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
0.105
-
 NAD+ formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
0.11
-
 NAD+ formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
0.13
-
 formate formate dehydrogenase II, reaction with NAD+ Cupriavidus oxalaticus
0.135
-
 formate formate dehydrogenase I, reaction with NAD+ Cupriavidus oxalaticus
0.2
-
 2,6-dichlorophenolindophenol formate dehydrogenase I or formate dehydrogenase II, reaction with NADH Cupriavidus oxalaticus
0.24
-
 benzyl viologen formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
0.25
-
 benzyl viologen formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
0.46
-
 ferricyanide formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
0.5
-
 ferricyanide formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
0.62
-
 ferricyanide formate dehydrogenase II, reaction with NADH Cupriavidus oxalaticus
0.69
-
 ferricyanide formate dehydrogenase I, reaction with NADH Cupriavidus oxalaticus
2
-
 benzyl viologen formate dehydrogenase II, reaction with NADH Cupriavidus oxalaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 1 * 59000 + 1 * 100000, formate dehydrogenase II, SDS-PAGE Cupriavidus oxalaticus
59000
-
 2 * 59000 + 2 * 100000, formate dehydrogenase I, SDS-PAGE Cupriavidus oxalaticus
100000
-
 1 * 59000 + 1 * 100000, formate dehydrogenase II, SDS-PAGE Cupriavidus oxalaticus
100000
-
 2 * 59000 + 2 * 100000, formate dehydrogenase I, SDS-PAGE Cupriavidus oxalaticus
175000
-
 formate dehydrogenase II, sucrose density gradient centrifugation Cupriavidus oxalaticus
315000
-
 formate dehydrogenase I, sucrose density gradient centrifugation Cupriavidus oxalaticus

Organism

Organism UniProt Comment Textmining
Cupriavidus oxalaticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cupriavidus oxalaticus

Reaction

Reaction Comment Organism Reaction ID
formate + NAD+ = CO2 + NADH + H+ sequential mechanism Cupriavidus oxalaticus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
-
 Cupriavidus oxalaticus

Storage Stability

Storage Stability Organism
0°C, under anaerobic conditions, stable for some weeks Cupriavidus oxalaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
 Cupriavidus oxalaticus 2 ferrocyanide + NAD+ + H+
-
 ?
formate + 2,6-dichloroindophenol
-
 Cupriavidus oxalaticus CO2 + reduced 2,6-dichlorophenolindophenol
-
 ?
formate + 2,6-dichlorophenolindophenol
-
 Cupriavidus oxalaticus CO2 + reduced 2,6-dichlorophenolindophenol
-
 r
formate + benzyl viologen
-
 Cupriavidus oxalaticus CO2 + reduced benzyl viologen
-
 ?
formate + FAD
-
 Cupriavidus oxalaticus CO2 + FADH2
-
 ?
formate + ferricyanide
-
 Cupriavidus oxalaticus CO2 + ferrocyanide
-
 ?
formate + FMN
-
 Cupriavidus oxalaticus CO2 + FMNH2
-
 ?
formate + methyl viologen
-
 Cupriavidus oxalaticus CO2 + reduced methyl viologen
-
 ?
formate + methylene blue
-
 Cupriavidus oxalaticus CO2 + reduced methylene blue
-
 ?
formate + NAD+
-
 Cupriavidus oxalaticus CO2 + NADH + H+
-
 ?
formate + nitrobluetetrazolium
-
 Cupriavidus oxalaticus CO2 + reduced nitrobluetetrazolium
-
 ?
formate + phenazine methosulfate
-
 Cupriavidus oxalaticus CO2 + reduced phenazine methosulfate
-
 ?
formate + riboflavin
-
 Cupriavidus oxalaticus CO2 + reduced riboflavin
-
 ?
NADH + 2,6-dichloroindophenol
-
 Cupriavidus oxalaticus NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
NADH + benzyl viologen
-
 Cupriavidus oxalaticus NAD+ + reduced benzyl viologen
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 59000 + 1 * 100000, formate dehydrogenase II, SDS-PAGE Cupriavidus oxalaticus
More formate dehydrogenase I is a dimer of formate dehydrogenase II Cupriavidus oxalaticus
tetramer 2 * 59000 + 2 * 100000, formate dehydrogenase I, SDS-PAGE Cupriavidus oxalaticus

Cofactor

Cofactor Comment Organism Structure
FMN formate dehydrogenase I contains 1 mol of FMN per mol of enzyme, formate dehydrogenase II contains 2 mol of FMN per mol of enzyme Cupriavidus oxalaticus
NAD+
-
 Cupriavidus oxalaticus