Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | 0.25 mM | Cupriavidus oxalaticus | |
N3- | 0.25 mM. Inhibition is partly overcome by addition of formate or NAD+ | Cupriavidus oxalaticus | |
NADH | inhibition is partly overcome by addition of formate or NAD+ | Cupriavidus oxalaticus | |
NO3- | 0.25 mM. Inhibition is partly overcome by addition of formate or NAD+ | Cupriavidus oxalaticus | |
p-hydroxymercuribenzoate | 0.25 mM | Cupriavidus oxalaticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
2,6-dichlorophenolindophenol | formate dehydrogenase I, reaction with formate | Cupriavidus oxalaticus | |
0.042 | - |
2,6-dichlorophenolindophenol | formate dehydrogenase II, reaction with formate | Cupriavidus oxalaticus | |
0.105 | - |
NAD+ | formate dehydrogenase I, reaction with formate | Cupriavidus oxalaticus | |
0.11 | - |
NAD+ | formate dehydrogenase II, reaction with formate | Cupriavidus oxalaticus | |
0.13 | - |
formate | formate dehydrogenase II, reaction with NAD+ | Cupriavidus oxalaticus | |
0.135 | - |
formate | formate dehydrogenase I, reaction with NAD+ | Cupriavidus oxalaticus | |
0.2 | - |
2,6-dichlorophenolindophenol | formate dehydrogenase I or formate dehydrogenase II, reaction with NADH | Cupriavidus oxalaticus | |
0.24 | - |
benzyl viologen | formate dehydrogenase II, reaction with formate | Cupriavidus oxalaticus | |
0.25 | - |
benzyl viologen | formate dehydrogenase I, reaction with formate | Cupriavidus oxalaticus | |
0.46 | - |
ferricyanide | formate dehydrogenase I, reaction with formate | Cupriavidus oxalaticus | |
0.5 | - |
ferricyanide | formate dehydrogenase II, reaction with formate | Cupriavidus oxalaticus | |
0.62 | - |
ferricyanide | formate dehydrogenase II, reaction with NADH | Cupriavidus oxalaticus | |
0.69 | - |
ferricyanide | formate dehydrogenase I, reaction with NADH | Cupriavidus oxalaticus | |
2 | - |
benzyl viologen | formate dehydrogenase II, reaction with NADH | Cupriavidus oxalaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
1 * 59000 + 1 * 100000, formate dehydrogenase II, SDS-PAGE | Cupriavidus oxalaticus |
59000 | - |
2 * 59000 + 2 * 100000, formate dehydrogenase I, SDS-PAGE | Cupriavidus oxalaticus |
100000 | - |
1 * 59000 + 1 * 100000, formate dehydrogenase II, SDS-PAGE | Cupriavidus oxalaticus |
100000 | - |
2 * 59000 + 2 * 100000, formate dehydrogenase I, SDS-PAGE | Cupriavidus oxalaticus |
175000 | - |
formate dehydrogenase II, sucrose density gradient centrifugation | Cupriavidus oxalaticus |
315000 | - |
formate dehydrogenase I, sucrose density gradient centrifugation | Cupriavidus oxalaticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus oxalaticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus oxalaticus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
formate + NAD+ = CO2 + NADH + H+ | sequential mechanism | Cupriavidus oxalaticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Cupriavidus oxalaticus |
Storage Stability | Organism |
---|---|
0°C, under anaerobic conditions, stable for some weeks | Cupriavidus oxalaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Cupriavidus oxalaticus | 2 ferrocyanide + NAD+ + H+ | - |
? | |
formate + 2,6-dichloroindophenol | - |
Cupriavidus oxalaticus | CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
formate + 2,6-dichlorophenolindophenol | - |
Cupriavidus oxalaticus | CO2 + reduced 2,6-dichlorophenolindophenol | - |
r | |
formate + benzyl viologen | - |
Cupriavidus oxalaticus | CO2 + reduced benzyl viologen | - |
? | |
formate + FAD | - |
Cupriavidus oxalaticus | CO2 + FADH2 | - |
? | |
formate + ferricyanide | - |
Cupriavidus oxalaticus | CO2 + ferrocyanide | - |
? | |
formate + FMN | - |
Cupriavidus oxalaticus | CO2 + FMNH2 | - |
? | |
formate + methyl viologen | - |
Cupriavidus oxalaticus | CO2 + reduced methyl viologen | - |
? | |
formate + methylene blue | - |
Cupriavidus oxalaticus | CO2 + reduced methylene blue | - |
? | |
formate + NAD+ | - |
Cupriavidus oxalaticus | CO2 + NADH + H+ | - |
? | |
formate + nitrobluetetrazolium | - |
Cupriavidus oxalaticus | CO2 + reduced nitrobluetetrazolium | - |
? | |
formate + phenazine methosulfate | - |
Cupriavidus oxalaticus | CO2 + reduced phenazine methosulfate | - |
? | |
formate + riboflavin | - |
Cupriavidus oxalaticus | CO2 + reduced riboflavin | - |
? | |
NADH + 2,6-dichloroindophenol | - |
Cupriavidus oxalaticus | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
NADH + benzyl viologen | - |
Cupriavidus oxalaticus | NAD+ + reduced benzyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 59000 + 1 * 100000, formate dehydrogenase II, SDS-PAGE | Cupriavidus oxalaticus |
More | formate dehydrogenase I is a dimer of formate dehydrogenase II | Cupriavidus oxalaticus |
tetramer | 2 * 59000 + 2 * 100000, formate dehydrogenase I, SDS-PAGE | Cupriavidus oxalaticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | formate dehydrogenase I contains 1 mol of FMN per mol of enzyme, formate dehydrogenase II contains 2 mol of FMN per mol of enzyme | Cupriavidus oxalaticus | |
NAD+ | - |
Cupriavidus oxalaticus |