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Literature summary for 1.17.1.8 extracted from

  • Coulter, C.V.; Gerrard, J.A.; Kraunsoe, J.A.E.; Pratt, A.J.
    Escherichia coli dihydrodipicolinate synthase and dihydrodipicolinate reductase: kinetic and inhibition studies of two putative herbicide targets (1999), Pestic. Sci., 55, 887-895.
No PubMed abstract available
Search for more literature for 1.17.1.8

Application

Application Comment Organism
agriculture potential herbicide target, leads for herbicide development are inhibitors: dipicolinic acid, isophthalic acid, and DELTA3-tetrahydroisophthalic acid Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
dapB gene, overexpression from plasmid in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate
-
 Escherichia coli
DELTA3-tetrahydroisophthalic acid moderate inhibition Escherichia coli
dipicolinate reversible, competitive versus substrate (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate Escherichia coli
isophthalic acid reversible, competitive versus substrate 2,3-dihydrodipicolinate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0066
-
 NADH
-
 Escherichia coli
0.0165
-
 NADPH
-
 Escherichia coli
0.529
-
 (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-dihydrodipicolinate + NAD(P)H Escherichia coli
-
 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpression in Escherichia coli from plasmid Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
135
-
 purified enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + NADPH + H+
-
 Escherichia coli ?
-
 r
2,3-dihydrodipicolinate + NAD(P)H
-
 Escherichia coli 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
? ? * 28757-29000, SDS-PAGE and sequence determination Escherichia coli

Synonyms

Synonyms Comment Organism
DHDPR
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Escherichia coli
NADPH
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.435
-
 (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate
-
 Escherichia coli
5.5
-
 isophthalic acid
-
 Escherichia coli