Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.1.5 extracted from

  • Nagel, M.; Andreesen, J.R.
    Purification and characterization of the molybdoenzymes nicotinate dehydrogenase and 6-hydroxynicotinate dehydrogenase from Bacillus niacini (1990), Arch. Microbiol., 154, 605-613.
No PubMed abstract available

General Stability

General Stability Organism
lyophilization completely inactivates, high protein concentration may have a stabilizing effect on enzyme activity, sugar protects enzyme from dissociation Neobacillus niacini

Inhibitors

Inhibitors Comment Organism Structure
KCN after removal of cyanide under anaerobic conditions, enzyme activity can be reconstituted by anaerobic incubation with Na2S Neobacillus niacini
Sulfide complete loss of activity without previous KCN-treatment Neobacillus niacini

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
nicotinate
-
Neobacillus niacini

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Neobacillus niacini
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE Neobacillus niacini
34000
-
x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE Neobacillus niacini
85000
-
x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE Neobacillus niacini
280000 340000 gel filtration, non-denaturing PAGE Neobacillus niacini

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinate + H2O + electron acceptor Neobacillus niacini first step of nicotinate catabolism 6-hydroxynicotinate + reduced electron acceptor
-
?

Organism

Organism UniProt Comment Textmining
Neobacillus niacini
-
DSM 2923
-

Purification (Commentary)

Purification (Comment) Organism
-
Neobacillus niacini

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.54
-
-
Neobacillus niacini

Storage Stability

Storage Stability Organism
-20°C, increase of activity in crude extract Neobacillus niacini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with Brilliant cresyl blue, cytochrome c, FAD, ferricyanide, FMN, menadione, NAD(P)+, and both viologens as electron acceptors, not as substrates: 2- or 6-aminonicotinamide, 2-aminonicotinic acid, chelidamic acid, cinchomeronic acid, citrazinic acid, dinicotinic acid, dipicolinic acid, 3-hydroxypicolinic acid, hypoxanthine, isocinchomeronic acid, isonicotinic acid, lutidinic acid, nicotinamide, xanthine Neobacillus niacini ?
-
?
nicotinate + H2O + electron acceptor equilibrium lies far in direction of 6-hydroxynicotinate formation Neobacillus niacini 6-hydroxynicotinate + reduced electron acceptor
-
r
nicotinate + H2O + electron acceptor high substrate specificity, transfers electrons only to artificial electron acceptors of high redox potential, i.e. thiazolyl blue, nitroblue tetrazolium chloride, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol, methylene blue with 40% of the activity with phenazine ethosulfate and 2,6-dichlorophenolindophenol Neobacillus niacini 6-hydroxynicotinate + reduced electron acceptor
-
r
nicotinate + H2O + electron acceptor first step of nicotinate catabolism Neobacillus niacini 6-hydroxynicotinate + reduced electron acceptor
-
?

Subunits

Subunits Comment Organism
oligomer x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE Neobacillus niacini

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Neobacillus niacini

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Neobacillus niacini

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center enzyme contains Fe/S centers, 8.3 mol iron per mol of enzyme, 1.5 mol acid-labile sulfur per mol of enzyme Neobacillus niacini
Bactopterin enzyme contains bactopterin, an eubacterial modification of molybdopterin, the organic moiety of the molybdenum cofactor Neobacillus niacini
FAD enzyme contains FAD, 2 mol flavin per mol of enzyme Neobacillus niacini
molybdenum cofactor enzyme contains 1.5 mol molybdenum per mol of enzyme and bactopterin Neobacillus niacini