Literature summary for 1.17.1.4 extracted from

Tramper, J.; Angelino, S.A.G.F.; Muller, F.; van der Plas, H.C.
Kinetics and stability of immobilized chicken liver xanthine dehydrogenase (1979), Biotechnol. Bioeng., 21, 1767-1786.

Search for more literature for 1.17.1.4

Application

Application	Comment	Organism
synthesis	immobilized xanthine dehydrogenase for use in organic synthesis	Gallus gallus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.017	-	xanthine	pH 7.9, increasing Km with increasing pH	Gallus gallus
0.019	-	NAD+	pH 7.9, pH dependence, increasing Km with increasing pH	Gallus gallus
0.035	-	xanthine	immobilized enzyme preparation, pH 7.9, pH dependence, minimum Km at pH 8.1, increasing values below and above	Gallus gallus
0.054	-	NAD+	immobilized enzyme preparation, pH 7.9, pH dependence, minimum Km at pH 8.1, increasing values below and above	Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
xanthine + NAD+ + H2O	Gallus gallus	-	urate + NADH	-	?

Organism

Organism	UniProt	Comment	Textmining
Gallus gallus	-	chicken	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized on n-octylamine-substituted Sepharose 4B	Gallus gallus
to homogeneity, chromatography techniques	Gallus gallus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Gallus gallus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
xanthine + NAD+ + H2O	-	Gallus gallus	urate + NADH	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Gallus gallus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4	-	free enzyme, half-life: 18 days, immobilized enzyme, half-life: 88 days	Gallus gallus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Gallus gallus

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Release 2023.1 (January 2023)