Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-4-hydroxypteridine-6-carboxyaldehyde | competitive inhibition of 2-amino-4-hydoxy-pterine oxidation, Ki: 0.000016 mM | Bos taurus | |
2-amino-4-hydroxypteridine-6-carboxyaldehyde | competitive inhibition of 2-amino-4-hydroxy-pterine oxidation, Ki: 0.00025 mM, non-competitive inhibition of xanthine oxidation, Ki: 0.00051 mM | Drosophila melanogaster | |
8-Azaguanine | competitive inhibition of 2-amino-4-hydroxypterine oxidation, Ki: 0.0012 mM | Bos taurus | |
8-Azaguanine | competitive inhibition of xanthine oxidation, Ki: 0.037 mM, non-competitive inhibition of 2-amino-4-hydroxy-pterine oxidation, Ki: 0.071 mM | Drosophila melanogaster | |
ammeline | competitive inhibition of 2-amino-4-hydroxy-pterine oxidation, Ki: 0.016 mM | Bos taurus | |
ammeline | competitive inhibition of xanthine oxidation, Ki: 0.021 mM, non-competitive inhibition of 2-amino-4-hydoxypterine oxidation, Ki: 0.045 mM | Drosophila melanogaster | |
pyridoxal | Ki for 2-amino-4-hydroxypterine oxidation: 0.08 mM at 30 and 50°C, competitive; Ki for xanthine oxidation: 0.05 mM at 30°C, 0.11 mM at 50°C, competitive | Drosophila melanogaster | |
xanthine | substrate inhibition above 0.05 mM, but in the presence of NAD+ | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00083 | - |
methylene blue | electron donor: 2-amino-4-hydroxy-pterine | Drosophila melanogaster | |
0.001 | - |
2-amino-4-hydroxypterin | - |
Bos taurus | |
0.0033 | - |
NAD+ | electron donor: 2-amino-4-hydroxypterine | Drosophila melanogaster | |
0.004 | - |
2-amino-4-hydroxypterin | pH 7.0, electron acceptor: methylene blue | Drosophila melanogaster | |
0.0071 | - |
2-amino-4-hydroxypterin | pH 8.0, electron acceptor: methylene blue | Drosophila melanogaster | |
0.011 | - |
2-amino-4-hydroxypterin | increased Km in the presence of pyridoxal | Drosophila melanogaster | |
0.018 | - |
xanthine | electron acceptor: NAD+, 30°C | Drosophila melanogaster | |
0.025 | - |
NAD+ | electron donor: xanthine | Drosophila melanogaster | |
0.036 | - |
xanthine | increased Km in the presence of pyridoxal at 30°C | Drosophila melanogaster | |
0.04 | - |
xanthine | electron acceptor: NAD+, 50°C | Drosophila melanogaster | |
0.059 | - |
xanthine | increased Km in the presence of pyridoxal at 50°C | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + NAD+ + H2O | Drosophila melanogaster | i.e. pterin | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxy-pterin + NAD+ + H2O | Bos taurus | i.e. pterin | isoxanthopterin + NADH | - |
? | |
xanthine + NAD+ + H2O | Drosophila melanogaster | - |
urate + NADH | - |
? | |
xanthine + NAD+ + H2O | Bos taurus | - |
urate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Drosophila melanogaster | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
milk | - |
Bos taurus | - |
whole body | five different forms in electrophoretic mobility, but no differences in kinetic constants | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + methylene blue + H2O | - |
Drosophila melanogaster | isoxanthopterin + reduced methylene blue | - |
? | |
2-amino-4-hydroxy-pterin + NAD+ + H2O | i.e. pterin | Drosophila melanogaster | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxy-pterin + NAD+ + H2O | i.e. pterin | Bos taurus | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxypterin + NAD+ + H2O | i.e. pterin | Drosophila melanogaster | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxypterin + NAD+ + H2O | i.e. pterin | Bos taurus | isoxanthopterin + NADH | - |
? | |
xanthine + NAD+ + H2O | - |
Drosophila melanogaster | urate + NADH | - |
? | |
xanthine + NAD+ + H2O | - |
Bos taurus | urate + NADH | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Drosophila melanogaster | |
NAD+ | - |
Bos taurus |