General Stability | Organism |
---|---|
complete loss of activity when stored below 0°C | Gottschalkia acidurici |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
methyl viologen | electron donor: xanthine | Gottschalkia acidurici | |
1.35 | - |
xanthine | electron acceptor: methyl viologen | Gottschalkia acidurici |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | non-heme iron-sulfur groups, 7.7 g-atom Fe, 7.5 g- atom S2- per mol enzyme | Gottschalkia acidurici | |
Iron-sulfur-center | 2 Fe2-S2-clusters | Gottschalkia acidurici | |
Mo | - |
Gottschalkia acidurici | |
molybdenum-center | 1.8 g-atom molybdenum per native molecule | Gottschalkia acidurici | |
Se | 0.13 g-atom selenium per native enzyme molecule | Gottschalkia acidurici | |
Tungsten | 0.12 g-atom tungsten per native enzyme molecule | Gottschalkia acidurici |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE | Gottschalkia acidurici |
53000 | - |
1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE | Gottschalkia acidurici |
56000 | - |
1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE | Gottschalkia acidurici |
83000 | - |
1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE | Gottschalkia acidurici |
110000 | - |
1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE | Gottschalkia acidurici |
224000 | - |
gel filtration | Gottschalkia acidurici |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + O2 + H2O | Gottschalkia acidurici | very low activity | urate + O2- + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gottschalkia acidurici | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques, preparative gel electrophoresis | Gottschalkia acidurici |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | selenium is needed for xanthine dehydrogenase formation | Gottschalkia acidurici | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
385 | - |
- |
Gottschalkia acidurici |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxypyrazolo(3,4-d)pyrimidine + methyl viologen + H2O | i.e. allopurinol | Gottschalkia acidurici | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced methyl viologen | - |
? | |
4-hydroxypyrazolo(3,4-d)pyrimidine + methyl viologen + H2O | 8% of the activity compared to xanthine | Gottschalkia acidurici | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced methyl viologen | - |
? | |
5-aminoimidazol-4-carboxamide + methyl viologen + H2O | 1.1% of the activity compared to xanthine | Gottschalkia acidurici | ? + reduced methyl viologen | - |
? | |
6-mercaptopurine + methyl viologen + H2O | 9.5% of the activity compared to xanthine | Gottschalkia acidurici | ? + reduced methyl viologen | - |
? | |
hypoxanthine + methyl viologen + H2O | 7% of the activity compared to xanthine | Gottschalkia acidurici | xanthine + reduced methyl viologen | - |
? | |
purine + methyl viologen + H2O | 2% of the activity compared to xanthine | Gottschalkia acidurici | ? + reduced methyl viologen | - |
? | |
xanthine + 2,6-dichlorophenolindophenol + H2O | 2.5% of the activity compared to methyl viologen as electron acceptor | Gottschalkia acidurici | urate + ? | - |
? | |
xanthine + benzyl viologen + H2O | 52% of the activity compared to methyl viologen as electron acceptor | Gottschalkia acidurici | urate + reduced benzyl viologen | - |
? | |
xanthine + ferricyanide + H2O | 99% of the activity compared to methyl viologen as electron acceptor | Gottschalkia acidurici | urate + ferrocyanide | - |
? | |
xanthine + methyl viologen + H2O | best substrates tested, no activity with NAD+ or NADP+, 40% of the activity in the reverse reaction | Gottschalkia acidurici | urate + reduced methyl viologen | - |
r | |
xanthine + methylene blue + H2O | 87% of the activity compared to methyl viologen as electron acceptor | Gottschalkia acidurici | urate + reduced methylene blue | - |
? | |
xanthine + nitroblue tetrazolium + H2O | 32% of the activity compared to methyl viologen as electron acceptor | Gottschalkia acidurici | urate + ? | - |
? | |
xanthine + O2 + H2O | very low activity | Gottschalkia acidurici | urate + O2- + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | 1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE | Gottschalkia acidurici |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Gottschalkia acidurici |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
optimum value in the reverse reaction with urate and reduced methyl viologen | Gottschalkia acidurici |
8.5 | - |
optimum value for oxidation of xanthine and reduction of methyl viologen | Gottschalkia acidurici |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1.69 mol FAD per mol enzyme | Gottschalkia acidurici | |
additional information | molybdoironflavoprotein: ratio of non-heme iron to acid-labile sulfur to FAD to molybdenum to tungsten to selenium is 7.7 : 7.5 : 1.7 : 1.8 : 0.12 : 0.13 | Gottschalkia acidurici |